Regulation of plant defense responses in Arabidopsis by EDR2, a PH and START domain-containing protein.
Regulation of plant defense responses in Arabidopsis by EDR2, a PH and START domain-containing protein.
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DOI：
10.1111/j.1365-313x.2005.02523.x
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发表时间：
2005-09
期刊：
影响因子：
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通讯作者：
Dingzhong Tang;J. Ade;C. Frye;R. Innes
中科院分区：
文献类型：
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作者：
Dingzhong Tang;J. Ade;C. Frye;R. Innes
我们已经确定了一个拟南芥突变体，显示增强的抗病性（edr 2）的活体营养型白粉病病原体白粉病菌。edr 2突变体叶片上的真菌生长抑制发生在感染过程的后期，并与接种后约5天坏死病变的形成相吻合。双突变体分析显示，edr 2介导的抗性受到抑制水杨酸（SA）诱导的防御信号的突变，包括npr 1，pad 4和pad 2，表明edr 2介导的抗病性是依赖于SA。然而，edr 2对细菌病原体假单胞菌pv.番茄DC 3000菌株。EDR 2似乎在所有组织和器官中组成型转录，并编码一种新的蛋白质，由推定的普列克底物蛋白同源（PH）结构域和类固醇生成急性调节蛋白相关的脂质转移（START）结构域组成，并含有N-末端线粒体靶向序列。PH和START结构域与脂质结合有关，表明EDR 2可能在脂质信号传导和线粒体介导的程序性细胞死亡激活之间提供联系。
We have identified an Arabidopsis mutant that displays enhanced disease resistance (edr2) to the biotrophic powdery mildew pathogen Erysiphe cichoracearum. Inhibition of fungal growth on edr2 mutant leaves occurred at a late stage of the infection process and coincided with formation of necrotic lesions approximately 5 days after inoculation. Double-mutant analysis revealed that edr2-mediated resistance is suppressed by mutations that inhibit salicylic acid (SA)-induced defense signaling, including npr1, pad4 and sid2, demonstrating that edr2-mediated disease resistance is dependent on SA. However, edr2 showed normal responses to the bacterial pathogen Pseudomonas syringae pv. tomato strain DC3000. EDR2 appears to be constitutively transcribed in all tissues and organs and encodes a novel protein, consisting of a putative pleckstrin homology (PH) domain and a steroidogenic acute regulatory protein-related lipid-transfer (START) domain, and contains an N-terminal mitochondrial targeting sequence. The PH and START domains are implicated in lipid binding, suggesting that EDR2 may provide a link between lipid signaling and activation of programmed cell death mediated by mitochondria.