Characterization of Gicerin/MUC18/CD146 in the rat nervous system
Characterization of Gicerin/MUC18/CD146 in the rat nervous system
复制标题
DOI：
10.1002/jcp.10413
复制
发表时间：
2004-03-01
影响因子：
5.6
通讯作者：
Miki, N
中科院分区：
文献类型：
--
作者：
Taira, E;Kohama, K;Miki, N
Gicerin是从鸡中分离的免疫球蛋白（IG）超家族的细胞粘附分子。它显示嗜同性和嗜异性结合活性，并有两种亚型。s-Gicerin具有较小的胞浆结构域和与L-Gicerin相同的胞外结构域，具有较强的细胞粘附活性。在鸡的神经系统中，gicerin的表达只在发育阶段观察到，当神经元延长轴突和迁移。在其他组织中，gicerin参与组织再生或肿瘤发生。在这份报告中，我们确定了两种亚型的大鼠gicerin对应的鸡，我们的结论是，gicerin是一个同源的人CD 146/MUC 18/MCAM。接下来，我们产生抗体来表征神经系统中的大鼠gicerin。Gicerin在成年大鼠的脊髓的海马细胞、浦肯野细胞和感觉神经元中表达，而在肺中表达最丰富。除此之外，其在海马中的表达通过电休克而增加，表明在成熟神经系统中的一些作用。我们还显示了gicerin对海马神经元突起的促进作用。(C)2003 Wiley-Liss，Inc.
Gicerin is a cell adhesion molecule of an immunoglobulin (Ig) superfamily isolated from a chicken. It shows homophilic and heterophilic binding activities and has two isoforms. s-Gicerin which has small cytoplasmic domain and the same extracellular domain as L-gicerin shows stronger cell adhesion activity. In the chick nervous system, gicerin expression is only observed in the developmental stage when neurons extend neurites and migrate. In other tissues, gicerin participates in the tissue regeneration or oncogenesis. In this report, we identified two isoforms of rat gicerin corresponding to chicken and we concluded that gicerin is a homologue of human CD146/MUC18/MCAM. Next we generated antibody to characterize a rat gicerin in the nervous system. Gicerin is expressed in the hippocampal cells, Purkinje cells, and sensory neurons of a spinal chord of an adult rat, while expressed most abundantly in the lung. In addition to this, its expression in the hippocampus was increased by electroconvulsive shock, suggesting some role in the mature nervous system. And we also showed neurite promotion activity of gicerin from hippocampal neurons. (C) 2003 Wiley-Liss, Inc.