Complete amino acid sequence of flavocytochrome b2 from baker's yeast.
Complete amino acid sequence of flavocytochrome b2 from baker's yeast.
复制标题
面包酵母黄细胞色素 b2 的完整氨基酸序列。
DOI：
10.1111/j.1432-1033.1985.tb09213.x
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
L. Peréz
中科院分区：
文献类型：
--
作者：
F. Lederer;S. Cortial;A. Bécam;P. Haumont;L. Peréz
酵母黄细胞色素b2的每个亚基都可以被蛋白酶选择性地切割成两个片段，氨基端片段α和羧基端片段β。前者的主要结构之前已有报道[Ghrir， B., Becam， A. M. & Lederer， F.(1984)]。[j].生物化学学报，2004,18(2):559 - 564。197-残基片段β的氨基酸序列现已确定。用溴化氰裂解；所得的三种多肽被金黄色葡萄球菌V8蛋白酶、糜凝胰蛋白酶和胰蛋白酶消化，有时经过琥珀酰化处理。完整的片段在柠檬酸酰化后也被提交给色氨酸裂解。肽通过薄层指纹或高压液相色谱分离。它们大多在液相测序仪中测序。成熟蛋白的511个残基氨基酸序列由此完全建立。二级结构预测表明，螺旋结构和伸展结构交替存在，前者的比例较高。与其他黄酮类蛋白的比较没有发现任何显著的序列相似性。
Each subunit of baker's yeast flavocytochrome b2 can be selectively cleaved by proteases into two fragments, amino-terminal fragment alpha and carboxy-terminal fragment beta. The primary structure of the former has been reported before [Ghrir, B., Becam, A. M. & Lederer, F. (1984) Eur. J. Biochem. 139, 59-74]. The amino acid sequence of the 197-residue fragment beta has now been established. The fragment was cleaved with cyanogen bromide; the three peptides thus obtained were submitted to digestions with Staphylococcus aureus V8 protease, chymotrypsin and trypsin, sometimes after succinylation. The complete fragment was also submitted to tryptic cleavage after citraconylation. Peptides were separated by thin-layer finger-printing or high-pressure liquid chromatography. They were mostly sequenced in a liquid-phase sequenator. The 511-residue amino acid sequence of the mature protein is thus completely established. Secondary structure predictions indicate an alternation of helical and extended structure, with a higher percentage of the former. Comparisons with other flavoproteins do not detect any significant sequence similarity.
影响因子：
2.9
作者：
P. Karplus;K. Walsh;J. Herriott
通讯作者：
P. Karplus;K. Walsh;J. Herriott
减少肽和蛋白质中的亚砜。
影响因子：
--
作者：
Houghten,RA;Li,CH
通讯作者：
Li,CH
猪肾D-氨基酸氧化酶的一级结构。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ronchi,S;Minchiotti,L;Galliano,M;Curti,B;Swenson,RP;WilliamsJr,CH;Massey,V
通讯作者：
Massey,V