Cytokine regulation of lactate dehydrogenase-elevating virus: inhibition of viral replication by interferon-gamma.
Cytokine regulation of lactate dehydrogenase-elevating virus: inhibition of viral replication by interferon-gamma.
复制标题
乳酸脱氢酶升高病毒的细胞因子调节：干扰素-γ抑制病毒复制。
DOI：
10.1016/0166-3542(94)90017-5
复制
发表时间：
1994
影响因子：
7.6
通讯作者：
Wong,GH
中科院分区：
文献类型：
--
作者：
Cafruny,WA;Bradley,SE;Broen,JJ;Wong,GH
乳酸脱氢酶升高病毒（LDV）是一种感染巨噬细胞的持久性鼠模型病毒，其复制的调控机制尚不清楚。本研究观察了小鼠重组γ-干扰素（IFN-γ）和肿瘤坏死因子-α（TNF-α）对LDV复制的影响。在细胞收获和体外LDV感染之前，从用IFN-γ处理的未感染小鼠获得的巨噬细胞中LDV容许性降低。当在巨噬细胞收获和LDV复制阳性细胞分析之前向新生LDV感染小鼠注射该细胞因子时，也观察到IFN-γ的病毒抑制。经TNF-α治疗后，持续性LDV感染小鼠的病毒血症水平升高。IFN-γ和TNF-α对体外培养的巨噬细胞中LDV复制均无任何直接影响，表明这些细胞因子的作用需要继发或辅助体内事件。这些结果提供了证据表明，LDV感染的干扰素介导的调节，并支持免疫系统在LDV-宿主关系中的作用。
The mechanisms which regulate the replication of lactate dehydrogenase-elevating virus (LDV), a persistent murine model virus which infects macrophages, are unclear. For this study, the effects of murine recombinant interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) on LDV replication were examined. LDV permissiveness was reduced in macrophages obtained from uninfected mice treated with IFN-γ prior to cell harvest and in vitro LDV infection. Virus inhibition by IFN-γ was also observed when neonatal LDV-infected mice were injected with this cytokine prior to macrophage harvest and analysis of LDV replication-positive cells. Persistently LDV-infected mice demonstrated an increase in viremia levels following treatment with TNF-α. Neither IFN-γ nor TNF-α had any direct in vitro effect on LDV replication in cultured macrophages, suggesting that the actions of these cytokines required secondary or accessory in vivo events. These results provide evidence for cytokine-mediated regulation of LDV infection and support a role for the immune system in the LDV-host relationship.