Temporal and Spatial Expression of KIF3B after Acute Spinal Cord Injury in Adult Rats
Temporal and Spatial Expression of KIF3B after Acute Spinal Cord Injury in Adult Rats
复制标题
成年大鼠急性脊髓损伤后 KIF3B 的时空表达
DOI：
10.1007/s12031-012-9901-7
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发表时间：
2013-02-01
影响因子：
3.1
通讯作者：
Zhang, Feng
中科院分区：
文献类型：
--
作者：
Yu, Xiaowei;Wen, Hai;Zhang, Feng
KIF3亚基KIF3B被证明与有丝分裂有关。已知它参与神经元的细胞内运输。为了阐明其在中枢神经系统的表达及其生物学功能，我们建立了大鼠急性脊髓挫伤模型。Western blot分析表明，脊髓损伤（SCI）后KIF3B的显著上调。免疫组化显示KIF3B在脊髓中广泛分布，包括神经元和胶质细胞。增殖细胞核抗原和表型特异性标记物的双重免疫荧光染色显示增殖的小胶质细胞和星形胶质细胞中KIF3B表达增加。我们的数据表明KIF 3B可能与SCI后小胶质细胞和星形胶质细胞的增殖有关。
The KIF3 subunit KIF3B was proved to be associated with mitosis. It has been known to be engaged in intracellular transport of neurons. To elucidate the certain expression and biological function in central nervous system, we performed an acute spinal cord contusion injury model in adult rats. Western blot analysis indicated a marked upregulation of KIF3B after spinal cord injury (SCI). Immunohistochemistry revealed wide distribution of KIF3B in spinal cord, including neurons and glial cells. Double immunofluorescent staining for proliferating cell nuclear antigen and phenotype-specific markers showed increases of KIF3B expression in proliferating microglia and astrocytes. Our data suggest that KIF3B may be implicated in the proliferation of microglia and astrocytes after SCI.