Estrogen receptor is associated with protein and phospholipid kinase activities.
Estrogen receptor is associated with protein and phospholipid kinase activities.
复制标题
雌激素受体与蛋白质和磷脂激酶活性相关。
DOI：
10.1016/0006-291x(86)90035-5
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Wittliff,JL
中科院分区：
文献类型：
--
作者：
Baldi,A;Boyle,DM;Wittliff,JL
用[Y-32 P]-ATP孵育MCF-7细胞，研究免疫纯化的雌激素受体进行自身磷酸化以及磷酸化磷脂酰肌醇和1,2 -二酰基甘油的能力。磷酸化产物的SDS凝胶电泳分析显示三个主要的标记磷酸肽，分子量分别为57、47和43千道尔顿。其他多肽也被检测到，尽管数量少得多。当在体外补充磷脂酰肌醇、磷脂酰肌醇-4- p或1,2 -二酰基甘油和32种p -脂质时，用薄层色谱法分析PI，而不是1,2 -二酰基甘油，部分转化为32 p -磷脂酸和磷脂酰肌醇-4-32 p。雌激素受体阴性细胞系（MDA）在与MCF-7细胞相同的条件下产生可忽略不计的32个p多肽和32个p脂质。
The ability of immunopurified estrogen receptor from MCF-7 cells, incubated with [Y-32 P]-ATP, to conduct its own phosphorylation and to phosphorylate phosphatidylinositol and 1, 2-diacylglycerol was investigated. SDS gel electrophoresis analysis of the phosphorylated products revealed three major labeled phosphopeptides with molecular weights of 57, 47 and 43 kilodaltons. Other polypeptides were detected although in much smaller amounts. When the phosphorylation system was supplemented in vitro with phosphatidylinositol, phosphatidylinositol-4-P, or 1, 2-diacylglycerol and the 32 P-lipids were analyzed by thin layer chromatography, PI, but not 1, 2-diacylglycerol, was converted in part to 32 P-phosphatidic acid and phosphatidylinositol-4-32 P. Phosphatidylinositol-4-P was also phosphorylated to phosphatidylinositol-4, 5-32 P. An estrogen receptor-negative cell line (MDA) produced negligible 32 P-polypeptides and 32 P-lipids under identical conditions used for MCF-7 cells.