APPLICATION OF SEQUENATOR ANALYSES TO STUDY OF PROTEINS
APPLICATION OF SEQUENATOR ANALYSES TO STUDY OF PROTEINS
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DOI：
10.1021/bi00774a011
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发表时间：
1972-01-01
期刊：
影响因子：
2.9
通讯作者：
ERICSSON, LH
中科院分区：
文献类型：
--
作者：
HERMODSON, MA;NEURATH, H;ERICSSON, LH
Mark A.作者声明：H. Ericsson，Koiti Titani，Hans Neurath，* 和Kenneth A.沃尔什 * 摘要：Edman和Begg（Edman，P.，和Begg，G.（1967），Ear. J. Biochem.1，80）已经被修改并应用于蛋白质和蛋白质片段。显著的修改包括更换Quadrol的挥发性缓冲液（二甲基苄基胺），硫醇的引入，以稳定反应产物，并通过自动气液色谱法的甲硅烷基化苯硫基乙内酰脲的反应产物的识别。通过这些和其他修改，30-50
Mark A. Hermodson, Lowell H. Ericsson, Koiti Titani, Hans Neurath,* and Kenneth A. Walsh* abstract: The method of sequenator analysis described by Edman andBegg (Edman, P., and Begg, G.(1967), Ear. J. Biochem. 1, 80) has been modified and applied to proteins and protein fragments. Significant modifications include the re-placement of Quadrol by a volatile buffer (dimethylbenzyl-amine), the introduction of thiols to stabilize the reaction products, and the identification of the reaction products as silylated phenylthiohydantoins by automated gas-liquid chromatography. With these andother modifications, 30-50