Antioxidant activities of extracts and fractions from Lysimachia foenum-graecum Hance
Antioxidant activities of extracts and fractions from Lysimachia foenum-graecum Hance
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DOI：
10.1016/j.biortech.2008.07.021
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发表时间：
2009-01-01
影响因子：
11.4
通讯作者：
Li, Jian-ping
中科院分区：
文献类型：
--
作者：
Li, Hai-yun;Hao, Zai-bin;Li, Jian-ping
本研究研究了薰衣草水提取物、甲醇提取物、乙酸乙酯和甲醇提取物的正丁醇部分的抗氧化活性。建立了体外系统中的各种方法，例如磷钼法测定总抗氧化能力、对1,1-二苯基-2-三硝基苯肼（DPPH）自由基、超氧阴离子自由基和羟基自由基的清除活性。提取物和级分中总酚和总黄酮的含量也通过分光光度法测定。结果表明，所有提取物和级分均表现出不同强度的抗氧化和自由基清除活性。四种方法测定的提取物的抗氧化和自由基清除活性由高到低的顺序为：乙酸乙酯提取物>正丁醇提取物>甲醇提取物>水提取物。总酚类和总类黄酮含量的这种相似顺序表明，抗氧化和自由基清除活性的程度与提取物和级分中存在的酚类和类黄酮的含量一致。茴香提取物可能是有价值的抗氧化天然来源，并且似乎适用于健康医学和食品工业。 (C) 2008 Elsevier Ltd. 保留所有权利。
The antioxidant activities of water extract, methanol extract, ethyl acetate, and n-BuOH fractions of methanol extract from Lysimachia foenum-graecum Hance were investigated in this study. Various methods, such as the total antioxidant capacity measured by phosphomolybdenum method, scavenging activities towards 1,1-diphenyl-2-picryhydrazyl (DPPH) radical, superoxide anion radical, and hydroxyl radical, were established in in vitro systems. The amounts of total phenolics and total flavonoids in the extracts and fractions were also determined by spectrophotometric methods. The results showed that all the extracts and fractions exhibited antioxidant and radical-scavenging activities at different magnitudes of potency. The decreasing order of antioxidant and radical-scavenging activities among the extracts assayed through all the four methods were found to be ethyl acetate fraction > n-BuOH fraction > methanol extract > water extract. This similar order of the amounts of total phenolics and total flavonoids shows that the extent of antioxidant and radical-scavenging activities is in accordance with the amounts of phenolics and flavonoids present in extracts and fractions. The extracts of L foenum-graecum Hance might be valuable antioxidant natural sources and seemed to be applicable in both healthy medicine and food industry. (C) 2008 Elsevier Ltd. All rights reserved.