Rapid Point-of-Care Assay by SERS Detection of SARS-CoV-2 Virus and Its Variants
Rapid Point-of-Care Assay by SERS Detection of SARS-CoV-2 Virus and Its Variants
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DOI：
10.1021/acs.analchem.2c03437
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发表时间：
2022-12-13
影响因子：
7.4
通讯作者：
Gu, Man-Man
中科院分区：
文献类型：
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作者：
Guan, Peng-Cheng;Zhang, Hong;Gu, Man-Man
应对2019冠状病毒病（COVID-19）的传播，强调了对快速，准确和低成本诊断方法的需求，这些方法可以检测SARS-CoV-2感染的特异性抗原。COVID-19的检测基于逆转录PCR（RT-PCR），需要实验室服务且耗时。在这里，通过靶向SARS-CoV-2刺突蛋白，我们提出了一个即时的护理SERS检测平台，通过捕获底物和基于适体特异性识别的检测探针，在一个步骤中特异性地检测SARS-CoV-2抗原。使用该假病毒，无需任何预处理，通过手持式拉曼光谱仪在5 min内检测SARS-CoV-2病毒及其变体。该假病毒的检测限（LoD）为124 TU mu L-1（18 fM刺突蛋白），线性范围为250 - 10，000 TU mu L-1。此外，该检测方法可以特异性识别SARS-CoV-2抗原，而不会与其他冠状病毒或甲型流感病毒的特异性抗原发生交叉反应。因此，该平台在SARS-CoV-2的快速床旁诊断检测中具有很大的应用潜力。
Addressing the spread of coronavirus disease 2019 (COVID-19) has highlighted the need for rapid, accurate, and low-cost diagnostic methods that detect specific antigens for SARS-CoV-2 infection. Tests for COVID-19 are based on reverse transcription PCR (RT-PCR), which requires laboratory services and is time-consuming. Here, by targeting the SARS-CoV-2 spike protein, we present a point-of-care SERS detection platform that specifically detects SARS-CoV-2 antigen in one step by captureing substrates and detection probes based on aptamer-specific recognition. Using the pseudovirus, without any pretreatment, the SARS-CoV-2 virus and its variants were detected by a handheld Raman spectrometer within 5 min. The limit of detection (LoD) for the pseudovirus was 124 TU mu L-1 (18 fM spike protein), with a linear range of 250-10,000 TU mu L-1. Moreover, this assay can specifically recognize the SARS-CoV-2 antigen without cross reacting with specific antigens of other coronaviruses or influenza A. Therefore, the platform has great potential for application in rapid point-of-care diagnostic assays for SARS-CoV-2.