Diet-related gut bacterial dysbiosis correlates with impaired development, increased mortality and Nosema disease in the honeybee (Apis mellifera)
Diet-related gut bacterial dysbiosis correlates with impaired development, increased mortality and Nosema disease in the honeybee (Apis mellifera)
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DOI：
10.1111/mec.13862
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发表时间：
2016-11-01
影响因子：
4.9
通讯作者：
Anderson, Kirk E.
中科院分区：
文献类型：
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作者：
Maes, Patrick W.;Rodrigues, Pedro A. P.;Anderson, Kirk E.
菌群失调，定义为细菌群落组成的不健康变化，可以降低肠道对内在病原体的定植抵抗力。在这里，我们确定了饮食年龄和类型对蜜蜂（意大利蜜蜂）的健康和细菌群落组成的影响。我们喂新出现的蜜蜂新鲜或老化的饮食，然后记录主机的发展和细菌群落组成从四个不同的区域主机的消化道。喂食新鲜花粉或新鲜替代品，我们没有发现宿主死亡率，饮食消费，发展或微生物群落组成的差异。相比之下，喂食老年饮食的蜜蜂发育受损，死亡率增加，并形成了显着的生态失调微生物组。老化饮食的摄入导致核心回肠细菌Snod-grassella alvi的显着减少，而内在病原体Frischella perrara的相应增加。此外，S.回肠中的ALVI与宿主的存活和发育呈正相关。F. perrara和Apium副沙杆菌。总的来说，我们的研究结果表明，S。alvi与健康的护士发展相关，并可能排除F。来自回肠的perrara和P. apium。虽然在低丰度，建立共同的中肠病原体微孢子虫属。与回肠生态失调和相关宿主缺陷显著相关。此外，回肠中的生态失调反映在直肠、口器和下咽腺中，表明系统性宿主效应。我们的研究结果表明，饮食质量的典型变化在菌落健康和建立生态失调的肠道微生物组中起着重要作用。
Dysbiosis, defined as unhealthy shifts in bacterial community composition, can lower the colonization resistance of the gut to intrinsic pathogens. Here, we determined the effect of diet age and type on the health and bacterial community composition of the honeybee (Apis mellifera). We fed newly emerged bees fresh or aged diets, and then recorded host development and bacterial community composition from four distinct regions of the hosts' digestive tract. Feeding fresh pollen or fresh substitute, we found no difference in host mortality, diet consumption, development or microbial community composition. In contrast, bees fed aged diets suffered impaired development, increased mortality and developed a significantly dysbiotic microbiome. The consumption of aged diets resulted in a significant reduction in the core ileum bacterium Snod-grassella alvi and a corresponding increase in intrinsic pathogen Frischella perrara. Moreover, the relative abundance of S. alvi in the ileum was positively correlated with host survival and development. The inverse was true for both F. perrara and Parasacharibacter apium. Collectively, our findings suggest that the early establishment of S. alvi is associated with healthy nurse development and potentially excludes F. perrara and P. apium from the ileum. Although at low abundance, establishment of the common midgut pathogen Nosema spp. was significantly associated with ileum dysbiosis and associated host deficiencies. Moreover, dysbiosis in the ileum was reflected in the rectum, mouthparts and hypopharyngeal glands, suggesting a systemic host effect. Our findings demonstrate that typically occurring alterations in diet quality play a significant role in colony health and the establishment of a dysbiotic gut microbiome.