Osteocyte density in woven bone
Osteocyte density in woven bone
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DOI：
10.1016/j.bone.2004.07.002
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发表时间：
2004-11-01
期刊：
影响因子：
4.1
通讯作者：
Schaffler, MB
中科院分区：
文献类型：
--
作者：
Hernandez, CJ;Majeska, RJ;Schaffler, MB
编织骨在组织生长过程中迅速形成，受伤后和对某些合成代谢刺激的反应。编织骨和板层骨之间的功能差异可能部分归因于骨细胞密度（每单位组织的细胞数）的差异。据估计，编织骨含有的骨细胞是板层骨的4 - 8倍，尽管支持这一论断的原始数据有限。鉴于最近的研究结果暗示骨细胞作为骨重建，骨形成和骨体积的调节因子，编织骨和板层骨之间骨细胞密度的巨大差异可能具有重要的后果。在这项研究中，我们比较了骨细胞陷窝密度（陷窝/mm（2）组织）在大鼠板层骨和编织骨在几种不同的情况下形成。我们发现大鼠板层皮质骨的腔隙密度（834 +/- 83 cells/mm（2），平均值+/- SD）与通过膜内成骨形成的骨膜编织骨没有显著差异，或者响应于机械载荷（921 +/- 204个细胞/mm（2））或骨折骨痂的骨膜支撑区（1138 +/- 168个细胞/mm（2））。与此相反，在骨折骨痂的中心（间隙）区域，软骨内衍生的编织骨的腔隙密度几乎高出100（1875 +/- 270个细胞/mm（2）），而生长板的初级海绵状骨的腔隙密度比板层皮质骨高40（1674 +/- 228个细胞/mm（2））高于板层松质骨（1189 +/- 164）。这些发现表明编织骨中的腔隙密度根据骨骼部位和发育史而变化，并且在邻近骨髓间隙的软骨内衍生编织骨中似乎升高。鉴于大量的证据支持骨细胞作为骨重建的局部启动者，我们认为骨陷窝密度增加的编织骨可能会加速重建。(C)2004爱思唯尔公司All rights reserved.
Woven bone forms rapidly during tissue growth, following injury and in response to certain anabolic stimuli. Functional differences between woven and lamellar bone may be due, in part, to differences in osteocyte density (cells per unit tissue). Woven bone has been estimated to contain four to eight times more osteocytes than lamellar bone, although primary data to support this assertion are limited. Given recent findings implicating osteocytes as regulators of bone remodeling, bone formation and bone volume, such large differences in osteocyte density between woven and lamellar bone may have important consequences. In this study, we compared the density of osteocyte lacunae (lacunae/mm(2) tissue) in rat lamellar bone with that in woven bone formed under several different circumstances. We found that the lacunar density of lamellar cortical bone in the rat (834 +/- 83 cells/mm(2), mean +/- SD) did not differ significantly from that of periosteal woven bone formed via intramembranous osteogenesis, either in response to mechanical loading (921 +/- 204 cells/mm(2)) or in the periosteal buttressing region of the fracture callus (1138 +/- 168 cells/mm(2)). In contrast, lacunar density of endochondrally derived woven bone in the center (gap) region of fracture callus was nearly 100% greater (1875 +/- 270 cells/mm(2)) than in lamellar cortical bone while lacunar density of primary spongiosa of the growth plate was 40% greater (1674 +/- 228 cells/mm(2)) than that in lamellar cancellous bone (1189 +/- 164). These findings demonstrate that lacunar density in woven bone varies depending on skeletal site and developmental history and appears to be elevated in endochondrally derived woven bone adjacent to marrow space. Given the considerable evidence supporting osteocytes as local initiators of bone remodeling, we suggest that woven bone with increased lacunar density may undergo remodeling at an accelerated rate. (C) 2004 Elsevier Inc. All rights reserved.