Development of a SERS-Based Rapid Vertical Flow Assay for Point-of Care Diagnostics
Development of a SERS-Based Rapid Vertical Flow Assay for Point-of Care Diagnostics
复制标题
DOI：
10.1021/acs.analchem.6b04710
复制
发表时间：
2017-02-07
影响因子：
7.4
通讯作者：
Brosseau, C. L.
中科院分区：
文献类型：
--
作者：
Clarke, O. J. R.;Goodall, B. L.;Brosseau, C. L.
护理点 (POC) 诊断测试平台是医疗保健行业中一个不断增长的领域，因为它们具有快速提供结果、易于使用、降低成本以及能够将患者与护理联系起来的优势。虽然许多 POC 测试基于色谱流分析技术，但该技术缺乏灵敏度，且多重分析和定量分析能力有限。最近的几份报告已经开始研究将色谱流平台与更先进的读出技术相结合的可行性，从而实现重要医疗保健指标的现场采集、存储和传输。正在探索的一项此类技术是表面增强拉曼光谱（SERS）。在这项工作中，SERS 首次与快速垂直流 (RVF) 免疫技术结合用于检测抗 HCV 抗体，以扩展这一商用诊断平台的功能。使用报告修饰的金纳米颗粒 (AuNP) 获得了高质量且可重复的 SERS 光谱。系列稀释研究表明，SERS 与 RVF 技术的结合显示出下一代 POC 诊断的巨大潜力。
Point-of-care (POC) diagnostic testing platforms are a growing sector of the healthcare industry as they offer the advantages of rapid provision of results, ease of use, reduced cost, and the ability to link patients to care. While many POC tests are based on chromatographic flow assay technology, this technology suffers from a lack of sensitivity along with limited capacity for multiplexing and quantitative analysis. Several recent reports have begun to investigate the feasibility of coupling chromatographic flow platforms to more advanced read-out technologies which in turn enable on-site acquisition, storage, and transmission of important healthcare metrics. One such technology being explored is surface-enhanced Raman spectroscopy or SERS. In this work, SERS is coupled for the first time to a rapid vertical flow (RVF) immunotechnology for detection of anti-HCV antibodies in an effort to extend the capabilities of this commercially available diagnostic platform. High-quality and reproducible SERS spectra were obtained using reporter-modified gold nanoparticles (AuNPs). Serial dilution studies indicate that the coupling of SERS with RVF technology shows enormous potential for next-generation POC diagnostics.