Detection of α-methylacyl-coenzyme-A racemase transcripts in blood and urine samples of prostate cancer patients
Detection of α-methylacyl-coenzyme-A racemase transcripts in blood and urine samples of prostate cancer patients
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DOI：
10.1007/bf03256217
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发表时间：
2006-01-01
影响因子：
4
通讯作者：
Houghton, Raymond L.
中科院分区：
文献类型：
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作者：
Zehentner, Barbara K.;Secrist, Heather;Houghton, Raymond L.
背景资料：α-甲酰基辅酶A消旋酶（AMACR）已被证明是前列腺癌细胞的高度特异性标志物，即使在恶性进展的最早阶段。它在恶性组织中以比前列腺特异性抗原（PSA）高得多的水平表达，并且在正常前列腺上皮中不以可感知的水平表达。在这项研究中，我们展示了定量检测AMACR转录在前列腺癌患者外周血中使用实时RT-PCR。此外，我们已经进行了一项试点研究，以证明这种技术的潜在应用，用于检测前列腺癌患者的尿液样本中的前列腺肿瘤细胞。方法：实时RT-PCR检测AMACR在前列腺癌患者的表达。来自163名患者的血液样本在疾病进展的不同阶段进行了测试，有或没有治疗。良性前列腺疾病和其他类型的癌症患者的血液标本也evaluated.Results：在28的58例样本中，已知转移性疾病的患者正在接受治疗，AMACR表达信号高于截止值检测，符合循环肿瘤细胞的存在。在88例假定器官局限性疾病患者中，有39例循环肿瘤细胞水平较低。AMACR RT-PCR与已知血清PSA值的比较表明，这些参数的组合显着增加了检测进行性疾病的灵敏度。在一项初步研究中，分析7例前列腺癌患者的尿液样本中，AMACR表达水平升高检测到尿沉渣中的6个阶段T1前列腺癌患者和一个患者的阶段T2前列腺癌。结论：在这项研究中提出的数据表明，AMA CR实时RT-PCR可能有助于前列腺癌的检测和分期。
Background: alpha-Methylacyl-coenzyme-A racemase (AMACR) has been shown to be a highly specific marker for prostate cancer cells, even in the earliest stages of malignant progression. It is expressed at much higher levels than prostate-specific antigen (PSA) in malignant tissues, and is not expressed at appreciable levels in normal prostatic epithelium. In this study, we demonstrate the quantitative detection of AMACR transcripts in peripheral blood of prostate cancer patients using real-time RT-PCR. In addition, we have undertaken a pilot study to demonstrate the potential application of this technique for the detection of prostate tumor cells in urine samples from patients with prostate cancer.Methods: A real-time RT-PCR assay was developed for detection of the expression of AMACR in prostate cancer patients. Blood samples from 163 patients were tested at various stages of disease progression, with or without therapy. Blood specimens from patients with benign prostate disorders and other types of cancer were also evaluated.Results: In 28 of 58 samples from patients with known metastatic disease who were undergoing treatment, an AMACR expression signal above the cut-off value was detected, consistent with the presence of circulating tumor cells. In 39 of 88 patients with presumptive organ-confined disease, there was evidence of low levels of circulating tumor cells. Comparison of AMACR RT-PCR with known serum PSA values indicated that a combination of these parameters significantly increased the sensitivity for detection of progressive disease. In a pilot study analyzing urine samples from seven prostate cancer patients, elevated AMACR expression levels were detected in the urine sediments of four of six stage-T1 prostate cancer patients and in the one patient with stage-T2 prostate cancer.Conclusion: The data presented in this study indicates that AMA CR real-time RT-PCR may aid in the detection and staging of prostate cancer.