IN-VITRO ESTABLISHMENT AND CHARACTERIZATION OF 2 ACQUIRED IMMUNODEFICIENCY SYNDROME-RELATED LYMPHOMA CELL-LINES (BC-1 AND BC-2) CONTAINING KAPOSIS SARCOMA-ASSOCIATED HERPESVIRUS-LIKE (KSHV) DNA-SEQUENCES
IN-VITRO ESTABLISHMENT AND CHARACTERIZATION OF 2 ACQUIRED IMMUNODEFICIENCY SYNDROME-RELATED LYMPHOMA CELL-LINES (BC-1 AND BC-2) CONTAINING KAPOSIS SARCOMA-ASSOCIATED HERPESVIRUS-LIKE (KSHV) DNA-SEQUENCES
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DOI：
10.1182/blood.v86.7.2708.bloodjournal8672708
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发表时间：
1995-10-01
期刊：
影响因子：
20.3
通讯作者：
CHANG, Y
中科院分区：
文献类型：
--
作者：
CESARMAN, E;MOORE, PS;CHANG, Y
最近在90%以上的获得性免疫缺陷综合征（AIDS）相关的卡波西肉瘤组织中发现了两个独特的DNA片段。序列分析表明，这些片段属于一种先前未鉴定的人类疱疹病毒，卡波济肉瘤相关疱疹病毒（KSHV）。这些片段也已在人类免疫缺陷病毒阳性患者的非霍奇金淋巴瘤亚组中鉴定;特别是在体腔淋巴瘤（AIDS-BCBL）中。我们已经建立了两个来源于AIDS-BCBL的细胞系，BC-1和BC-2，它们保留了KSHV序列，并使用它们来进一步表征这种推定的病毒基因组。在这份报告中，我们证明了KSHV序列代表了一个更大的DNA分子的一部分，主要位于受感染细胞的细胞核中。从这两个细胞系的中期传播原位杂交显示这些序列的附加体结构。它们在细胞中作为大的核附加体的存在支持了先前的序列同源性数据，表明KSHV属于疱疹病毒家族。这些细胞系提供了KSHV序列的连续来源。因此，他们代表了一个重要的工具，为未来的研究，这种最近描述的人类疱疹病毒，可能参与卡波西肉瘤和一些艾滋病相关的非霍奇金淋巴瘤的发病机制。(C)1995年，美国血液学会。
Two unique DNA fragments were recently identified in over 90% of acquired immunodeficiency syndrome (AIDS)-related Kaposi's sarcoma tissues. Sequence analysis suggests that these fragments belong to a previously unidentified human herpesvirus, Kaposi's sarcoma-associated herpesvirus (KSHV). These fragments have also been identified in a subset of non-Hodgkin's lymphomas in human immunodeficiency virus-positive patients; specifically, in body cavity-based lymphomas (AIDS-BCBLs). We have established two cell lines derived from AIDS-BCBLs, BC-1 and BC-2, which retain the KSHV sequences, and have used them to further characterize this putative viral genome. In this report, we demonstrate that the KSHV sequences represent a portion of a much larger DNA molecule that is located predominantly in the nucleus of the infected cells. In situ hybridization of metaphase spreads made from both of these cell lines show these sequences in episomal structures. Their presence in the cells as large nuclear episomes supports previous sequence homology data suggesting that KSHV belongs to the herpesvirus family. These cell lines provide a continuous source of KSHV sequences. Thus, they represent an important tool for future studies of this recently described human herpesvirus that may be involved in the pathogenesis of Kaposi's sarcoma and some AIDS-related non-Hodgkin's lymphomas. (C) 1995 by The American Society of Hematology.