Inhibition of matrix metalloproteinase activity in human dentin via novel antibacterial monomer.
Inhibition of matrix metalloproteinase activity in human dentin via novel antibacterial monomer.
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DOI：
10.1016/j.dental.2014.12.011
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发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Xu HH
中科院分区：
文献类型：
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作者：
Li F;Majd H;Weir MD;Arola DD;Xu HH
牙本质-复合体粘结失效是由混合层降解等因素引起的，而混合层降解又可以由牙本质中暴露的胶原蛋白的水解和酶降解引起。本研究的目的是研究一种新型的抗菌单体甲基丙烯酸二甲氨基十二酯(DMADDM)作为基质金属蛋白酶(MMPs)的抑制剂，以及DMADDM对可溶性重组人MMPs和牙本质基质结合的内源性MMPs的影响。采用比色法检测不同质量分数(0.1%~10%)的DMADDM对可溶性rh MMP8和rh MMP9的抑制作用。脱矿人牙本质中基质结合的内源性基质金属蛋白酶活性被评估。将牙本质梁分为4组(n=10)，分别在含钙、锌的培养液(对照组)、对照组+0.2%洗必泰(CHX)、5%12-甲基丙烯酰氧基十二烷基溴化铵(MDPB)和5%DMADDM中孵育。通过三点弯曲力学测试、牙本质质量损失和羟脯氨酸测定来评价牙本质胶原肽的溶解情况。0.1%~10%DMADDM具有较强的抗基质金属蛋白酶活性，浓度为5%时，对重组人基质金属蛋白酶-8和9的抑制率达90%。与对照组相比，在含5%DMADDM的培养液中，牙本质梁的弹性模量值下降了34%(对照组下降了73%)，干性牙本质质量下降了3%(对照组下降了28%)，溶解的羟脯氨酸显著减少(p<；0.05)。新型抗菌单体DMADDM对可溶性重组人MMPs和结合基质的人牙本质MMPs均有明显的抑制作用。这些结果，再加上之前的研究表明，含有DMADDM的粘结剂在不影响牙本质粘结强度的情况下抑制了生物膜，表明DMADDM在粘结剂中有希望用于防止混合层中的胶原降解和保护树脂-牙本质粘结。
Dentin-composite bond failure is caused by factors including hybrid layer degradation, which in turn can be caused by hydrolysis and enzymatic degradation of the exposed collagen in the dentin. The objectives of this study were to investigate a new antibacterial monomer (dimethylaminododecyl methacrylate, DMADDM) as an inhibitor for matrix metalloproteinases (MMPs), and to determine the effects of DMADDM on both soluble recombinant human MMPs (rhMMPs) and dentin matrix-bound endogenous MMPs. Inhibitory effects of DMADDM at six mass% (0.1% to 10%) on soluble rhMMP-8 and rhMMP-9 were measured using a colorimetic assay. Matrix-bound endogenous MMP activity was evaluated in demineralized human dentin. Dentin beams were divided into four groups (n = 10) and incubated in calcium- and zinc-containing media (control medium); or control medium + 0.2% chlorhexidine (CHX); 5% 12-methacryloyloxydodecylpyridinium bromide (MDPB); or 5% DMADDM. Dissolution of dentin collagen peptides was evaluated by mechanical testing in three-point flexure, loss of dentin mass, and a hydroxyproline assay. Use of 0.1% to 10% DMADDM exhibited a strong concentration-dependent anti-MMP effect, reaching 90% of inhibition on rhMMP-8 and rhMMP-9 at 5% DMADDM concentration. Dentin beams in medium with 5% DMADDM showed 34% decrease in elastic modulus (vs. 73% decrease for control), 3% loss of dry dentin mass (vs. 28% loss for control), and significantly less solubilized hydroxyproline when compared with control (p < 0.05). The new antibacterial monomer DMADDM was effective in inhibiting both soluble rhMMPs and matrix-bound human dentin MMPs. These results, together with previous studies showing that adhesives containing DMADDM inhibited biofilms without compromising dentin bond strength, suggest that DMADDM is promising for use in adhesives to prevent collagen degradation in hybrid layer and protect the resin-dentin bond.