Block in anaphase chromosome separation caused by a telomerase template mutation
Block in anaphase chromosome separation caused by a telomerase template mutation
复制标题
DOI：
10.1126/science.275.5305.1478
复制
发表时间：
1997-03-07
期刊：
影响因子：
56.9
通讯作者：
Blackburn, EH
中科院分区：
文献类型：
--
作者：
Kirk, KE;Harmon, BP;Blackburn, EH
端粒对于染色体的稳定性是必不可少的，但它们在特定细胞周期阶段的功能尚不清楚。端粒现在被证明在有丝分裂过程中染色体分离中起作用。在嗜热四膜虫的端粒DNA突变体中，通过改变模板序列表达端粒酶RNA而产生，生殖细胞核的分裂在后期严重延迟或受阻，突变的染色单体不能在中间区完全分离，变得拉伸到其正常长度的两倍。这些结果表明突变体端粒分离的物理阻断。
Telomeres are essential for chromosome stability, but their functions at specific cell-cycle stages are unknown. Telomeres are now shown to have a role in chromosome separation during mitosis. In telomeric DNA mutants of Tetrahymena thermophila, created by expression of a telomerase RNA with an altered template sequence, division of the germline nucleus was severely delayed or blocked in anaphase, The mutant chromatids failed to separate completely at the midzone, becoming stretched io up to twice their normal length. These results suggest a physical block in mutant telomere separation.