A non-allogeneic stimulus triggers the production of de novo HLA antibodies in healthy adults
A non-allogeneic stimulus triggers the production of de novo HLA antibodies in healthy adults
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DOI：
10.1016/j.trim.2007.06.001
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发表时间：
2007-11-01
影响因子：
1.5
通讯作者：
Crispin, Jose C.
中科院分区：
文献类型：
--
作者：
Alberu, Josefina;Morales-Buenrostro, Luis E.;Crispin, Jose C.
背景：移植受者产生针对广泛的人类白细胞抗原特异性的抗体，而不仅仅是针对他们所接触的抗原。本研究的目的是确定人类白细胞抗原无关的免疫刺激是否能够触发人类白细胞抗原抗体的产生。方法：我们检测了20名健康成人在接受免疫刺激(乙肝疫苗加结核菌素皮肤试验)前后的人类白细胞抗原抗体的存在。结果：基线时，75%的受试者在保护性滴度中检测到了乙肝表面抗原抗体；除一人外，所有受试者均为阴性。抗原刺激后1周，乙肝表面抗原抗体滴度显著升高，但未检测到任何可检测到的人类白细胞抗原抗体的变化。令人惊讶的是，在施加刺激一个月后，8名参与者中出现了人类白细胞抗原抗体。一个月后，又有一名受试者出现了人类白细胞抗原抗体。因此，9/20名受试者在观察期内PRA转为阳性。用单抗原法鉴定抗体的特异性。免疫刺激诱导的同种异体抗体反应与记忆细胞刺激不一致。结论：本研究结果表明，非人类白细胞抗原刺激能够诱导健康成人产生人类白细胞抗原抗体。(C)2007 Elsevier B.V.保留所有权利。
Background: Transplant recipients develop antibodies against a wide array of HLA specificities, and not only against the antigens to which they have been exposed. The aim of the present study was to establish if HLA-unrelated immune stimulation is capable of triggering the production of HLA antibodies.Methods: We determined the presence of HLA antibodies in 20 healthy adults before and after the administration of an immune stimulus (hepatitis B vaccine plus tuberculin skin test). HLA antibodies were assessed with a flow-cytometry based system.Results: At baseline, HBsAg antibodies were detected in protective titers in 75% of the subjects; HLA antibodies were negative in all but one. One week after the antigenic stimulus, HBsAg antibody titers increased significantly, without any detectable changes in HLA antibodies. Surprisingly, HLA antibodies developed in 8 participants one month after the application of the stimulus. One additional subject developed HLA antibodies one month later. Therefore 9/20 subjects became PRA positive during the observation period. Antibody specificities were identified by single antigen assay. The alloantibody response elicited by the immune stimulus was not consistent with memory cell stimulation.Conclusions: The findings of this study demonstrate that a non-HLA antigenic stimulus is capable of eliciting the development of HLA antibodies in healthy adults. (c) 2007 Elsevier B.V. All rights reserved.