Increased initiation and growth of tumor cell lines, cancer stem cells and biopsy material in mice using basement membrane matrix protein (Cultrex or Matrigel) co-injection
Increased initiation and growth of tumor cell lines, cancer stem cells and biopsy material in mice using basement membrane matrix protein (Cultrex or Matrigel) co-injection
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DOI：
10.1038/nprot.2012.053
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发表时间：
2012-06-01
期刊：
影响因子：
14.8
通讯作者：
Bonfil, R. Daniel
中科院分区：
文献类型：
--
作者：
Fridman, Rafael;Benton, Gabriel;Bonfil, R. Daniel
该方案需要 2-4 小时，并提出了一种将肿瘤细胞、癌症干细胞或分散的活检材料注射到受体小鼠皮下或原位位置的方法。将肿瘤细胞或活检组织与基底膜基质蛋白（CultrexBME 或 Matrigel）在 4°C 下混合，然后注射到受体动物的首选解剖部位。肿瘤细胞还可以与其他细胞类型共同注射，例如成纤维细胞、基质细胞、内皮细胞等。详细介绍了适当的细胞数量、基底膜蛋白的处理和浓度、受体动物、注射位置和技术。该过程使得肿瘤能够从细胞或活检材料（肿瘤移植物）中生长，具有更高的获取和生长效率，并保留基于组织学的原发肿瘤表型。与其他细胞类型的共注射提供了更多的人类癌症生理模型，可用于药物筛选和癌症生物学研究。
This protocol requires 2-4 h and presents a method for injecting tumor cells, cancer stem cells or dispersed biopsy material into subcutaneous or orthotopic locations within recipient mice. The tumor cells or biopsy are mixed with basement membrane matrix proteins (CultrexBME or Matrigel) at 4 degrees C and then injected into recipient animals at preferred anatomical sites. Tumor cells can also be co-injected with additional cell types, such as fibroblasts, stromal cells, endothelial cells and so on. Details are given on appropriate cell numbers, handling and concentration of the basement membrane proteins, recipient animals, injection location and techniques. This procedure enables the growth of tumors from cells or biopsy material (tumor graft) with greater efficiency of take and growth, and with retention of the primary tumor phenotype based on histology. Co-injection with additional cell types provides more physiological models of human cancers for use in drug screening and studying cancer biology.