Reagent-loaded cartridges for valveless and automated fluid delivery in microfluidic devices
Reagent-loaded cartridges for valveless and automated fluid delivery in microfluidic devices
复制标题
DOI：
10.1021/ac049071x
复制
发表时间：
2005-01-01
影响因子：
7.4
通讯作者：
Whitesides, GM
中科院分区：
文献类型：
--
作者：
Linder, V;Sia, SK;Whitesides, GM
生命科学和医疗保健中的一个重要问题是简单快速地检测生物标志物。虽然微流体装置在解决这个问题上有潜在的用处，但目前用于自动化流体输送的技术（包括阀门和电渗透）需要复杂的芯片微加工、笨重的仪器或两者兼而有之。在本文中，我们描述了一种简单而可靠的技术，用于存储和提供一系列的试剂的微流控装置。该技术是低成本的，需要最少的用户干预，并且可以在资源贫乏的环境中执行（例如，在没有电力和计算机控制设备的情况下（实验室外）。在该方法中，通过顺序注入由空气间隔物分隔的试剂塞来填充由市售管道制成的药筒。空气隔片可防止试剂在检测盒制备、储存和使用期间相互混合。作为一个例子，我们使用这种“插入式盒”技术在2分钟内完成了微通道中的固相免疫测定，具有低纳摩尔的灵敏度，并在13分钟内证明了HIV的诊断。
An important problem in the life sciences and in health care is simple and rapid detection of biomarkers. Although microfluidic devices are potentially useful in addressing this problem, current techniques for automating fluid delivery-which include valves and electro-osmosis-require sophisticated microfabrication of the chip, bulky instrumentation, or both. In this paper, we describe a simple and reliable technique for storing and delivering a sequence of reagents to a microfluidic device. The technique is low-cost, requires minimal user intervention, and can be performed in resource-poor settings (e.g., outside of a laboratory) in the absence of electricity and computer-controlled equipment. In this method, cartridges made of commercially available tubing are filled by sequentially injecting plugs of reagents separated by air spacers. The air spacers prevent the reagents from mixing with each other during cartridge preparation, storage, and usage. As an example, we used this "plug-in cartridge" technology to complete a solid-phase immunoassay in a microchannel in 2 min with low-nanomolar sensitivity and demonstrate the diagnosis of HIV in 13 min.