Effect of platelet-rich plasma on migration and proliferation of SaOS-2 osteoblasts:: role of platelet-derived growth factor and transforming growth factor-β
Effect of platelet-rich plasma on migration and proliferation of SaOS-2 osteoblasts:: role of platelet-derived growth factor and transforming growth factor-β
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DOI：
10.1111/j.1743-6109.2006.00110.x
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发表时间：
2006-03-01
影响因子：
2.9
通讯作者：
Sacchi, MC
中科院分区：
文献类型：
--
作者：
Celotti, F;Colciago, A;Sacchi, MC
富血小板血浆（PRP）在治疗中用作骨折和伤口愈合中生长因子的来源;然而，关于其在愈合过程的不同方面的作用的数据很少。使用人成骨细胞系SaOS-2体外评价PRP和本制剂中存在的两种主要生长因子（血小板衍生生长因子[PDGF]和转化生长因子-β [TGF-β]）的作用，逆转录-聚合酶链反应显示SaOS-2表达PDGF-α和-β受体。向SaOS-2细胞中加入不同浓度的巴曲碱活化PRP，以评估细胞迁移（通过微量趋化性测定）和细胞增殖（通过[H-3]-胸苷掺入DNA）。用抗PDGF-β或抗TGF-β抗体进行免疫中和可以评估这些生长因子的特定作用。获得的总体结果表明，PRP剂量依赖性刺激趋化性和细胞增殖。PDGF和TGF-β似乎对这两个参数产生不同的影响，前者参与刺激细胞迁移，后者抑制细胞增殖。得出的结论是，不同的生长因子存在于激活的PRP可以具体有助于组织再生的主要过程。
Platelet-enriched plasma (PRP) is used in therapy as a source of growth factors in bone fracture and wound healing; however, few data exist on its role in the different aspects of the healing process. The effect of PRP and of the two main growth factors present in this preparation (platelet-derived growth factor [PDGF] and transforming growth factor-beta [TGF-beta]) was evaluated in vitro using the human osteoblastic cell line SaOS-2, which was shown by reverse transcription-polymerase chain reaction to express both PDGF-alpha and -beta receptors. Batroxobine-activated PRP was added in different concentrations to SaOS-2 cells to assess cell migration (by a microchemotaxis assay) and cell proliferation (by [H-3]-thymidine incorporation into the DNA). Immunoneutralization with anti-PDGF-beta or anti-TGF-beta antibodies allowed the assessment of the specific role of these growth factors. The overall results obtained indicate that PRP dose-dependently stimulates both chemotaxis and cell proliferation. PDGF and TGF-beta appear to exert distinct effects on the two parameters, the former involved in stimulating cell migration and the latter in inhibiting cell proliferation. It is concluded that the different growth factors present in activated PRP can specifically contribute to the main processes of tissue regeneration.