Coexistence and upregulation of three types of opioid receptors, mu, delta and kappa, in human hypertrophic scars
Coexistence and upregulation of three types of opioid receptors, mu, delta and kappa, in human hypertrophic scars
复制标题
人类增生性疤痕中三种阿片受体 mu、delta 和 kappa 的共存和上调
DOI：
10.1111/j.1365-2133.2008.08449.x
复制
发表时间：
2008-04-01
影响因子：
10.3
通讯作者：
Li, J-F.
中科院分区：
文献类型：
--
作者：
Cheng, B.;Liu, H-W.;Li, J-F.
背景 瘙痒或伤害性疼痛是肥厚性疤痕的一个重要临床问题。最近出现的证据表明阿片受体（OR）可能与皮肤感觉异常有关；然而，对于ORs在肥厚性疤痕局部无感中的病理生理作用知之甚少。 目的 研究ORs在正常人皮肤和肥厚性疤痕无感中的表达谱。方法 对10例初诊肥厚性疤痕无感患者和10例健康人进行皮肤活检。对这些皮肤组织的一部分进行角质形成细胞和成纤维细胞的原代培养。通过免疫荧光染色检查 OR 的定位，并通过实时聚合酶链反应 (PCR) 进行 OR 定量。结果免疫荧光染色显示 OR 类型 mu (MOR)、delta (DOR) 和 kappa (KOR) 共表达，主要定位于角质形成细胞和成纤维细胞样细胞。实时PCR结果显示，与正常皮肤相比，增生性疤痕中MOR、DOR和KOR的表达增强。与皮肤活检的结果一致，我们观察到与正常皮肤来源的培养的增生性疤痕角质形成细胞和成纤维细胞相比，MOR、DOR 和 KOR 的表达增强。结论我们的结果表明，三种类型的 OR（MOR、DOR 和 KOR）的表达在人类肥厚性疤痕中显着上调，表明 OR 上调与肥厚性疤痕中的局部麻木感之间可能存在联系。
Background Pruritus or nociceptive pain is a significant clinical problem of hypertrophic scars. Recently emerging evidence has indicated the possible involvement of opioid receptors (ORs) in abnormal cutaneous sensation; however, little is known about the pathophysiological role of ORs in local cacaesthesia in hypertrophic scars.Objectives To study the expression profile of ORs in normal human skin and hypertrophic scars with cacaesthesia.Method Skin biopsy was performed in 10 patients newly diagnosed as having hypertrophic scars with cacaesthesia, and in 10 healthy individuals. Parts of these skin tissues were subjected to primary culture of keratinocytes and fibroblasts. Localization of ORs was examined by immunofluorescence staining and quantitation of ORs was performed by real-time polymerase chain reaction (PCR).Results Immunofluorescence staining revealed that OR types mu (MOR), delta (DOR) and kappa (KOR) were coexpressed and located mainly in the keratinocytes and fibroblast-like cells. Real-time PCR indicated that the expression of MOR, DOR and KOR in hypertrophic scars was enhanced in comparison with normal skin. Consistent with the results from skin biopsy, we observed enhanced expression of MOR, DOR and KOR in the cultured keratinocytes and fibroblasts derived from hypertrophic scars in comparison with those derived from normal skin.Conclusions Our results demonstrate that expression of three types of ORs, MOR, DOR and KOR, was markedly upregulated in human hypertrophic scars, suggesting a possible link between upregulated ORs and local cacaesthesia in hypertrophic scars.