Aligned and random nanofibrous substrate for the in vitro culture of Schwann cells for neural tissue engineering
Aligned and random nanofibrous substrate for the in vitro culture of Schwann cells for neural tissue engineering
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DOI：
10.1016/j.actbio.2009.01.039
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发表时间：
2009-09-01
影响因子：
9.7
通讯作者：
Ramakrishna, S.
中科院分区：
文献类型：
--
作者：
Gupta, Deepika;Venugopal, J.;Ramakrishna, S.
周围神经组织工程目前的挑战是生产一种能够桥接长神经间隙的可植入支架，其将产生类似于自体移植物的结果，而不需要收获自体供体组织。定向和随机聚己内酯/明胶（PCL/明胶）纳米纤维支架制造的许旺细胞，在神经组织工程中，协助指导再生轴突的生长在体外培养。通过电纺聚合物共混物（PCL/明胶）获得的平均纤维直径范围为232 +/- 194至160 +/- 86 nm，具有高孔隙率（90%）。将PCL与明胶共混导致纳米纤维支架的亲水性增加，并产生更好的机械性能，接近PCL纳米纤维。通过MTS试验评估所制造的纳米纤维的生物相容性用于培养和增殖雪旺细胞。MTS测定和扫描电子显微镜的结果证实，对齐和随机的PCL/明胶纳米纤维支架是合适的基板相比，神经组织工程的PCL纳米纤维支架的许旺细胞生长。(C)2009 Acta Materialia。Inc.由爱思唯尔有限公司出版。保留所有权利。
The current challenge in peripheral nerve tissue engineering is to produce an implantable scaffold capable of bridging long nerve gaps that will produce results similar to autograft without requiring the harvest of autologous donor tissue. Aligned and random polycaprolactone/gelatin (PCL/gelatin) nanofibrous scaffolds were fabricated for the in vitro culture of Schwann cells that assist in directing the growth of regenerating axons in nerve tissue engineering. The average fiber diameter attained by electrospinning of polymer blend (PCL/gelatin) ranged from 232 +/- 194 to 160 +/- 86 nm with high porosity (90%). Blending PCL with gelatin resulted in increased hydrophilicity of nanofibrous scaffolds and yielded better mechanical properties, approaching those of PCL nanofibers. The biocompatibility of fabricated nanofibers was assessed for culturing and proliferation of Schwann cells by MTS assay. The results of the MTS assay and scanning electron microscopy confirmed that aligned and random PCL/gelatin nanofibrous scaffolds are suitable substrates for Schwann cell growth as compared to PCL nanofibrous scaffolds for neural tissue engineering. (C) 2009 Acta Materialia. Inc. Published by Elsevier Ltd. All rights reserved.