Protective Effects of d-Limonene on Lipid Peroxidation and Antioxidant Enzymes in Streptozotocin-Induced Diabetic Rats
Protective Effects of d-Limonene on Lipid Peroxidation and Antioxidant Enzymes in Streptozotocin-Induced Diabetic Rats
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DOI：
10.1111/bcpt.12010
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发表时间：
2013-03-01
影响因子：
3.1
通讯作者：
Saravanan, Ramalingam
中科院分区：
文献类型：
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作者：
Murali, Ramakrishnan;Karthikeyan, Arumugam;Saravanan, Ramalingam
本研究的目的是评估d-柠檬烯对正常和链脲佐菌素（STZ）诱导的糖尿病大鼠血浆和组织中脂质过氧化副产物水平和抗氧化防御系统的保护作用。采用STZ单剂量（40mg/kg i.p）诱导大鼠实验性糖尿病，d-柠檬烯持续治疗45d。处理期后，氧化应激参数如脂质过氧化副产物；酶促抗氧化剂，如超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽s -转移酶；测定实验大鼠血浆和组织中还原性谷胱甘肽、维生素C和维生素E等非酶抗氧化剂的含量。在未经治疗的糖尿病大鼠中观察到脂质过氧化副产物水平的增加和抗氧化酶的显著降低。与未治疗的糖尿病组相比，给糖尿病大鼠服用d-柠檬烯45天，导致脂质过氧化副产物水平显著降低，抗氧化酶活性增加。与正常大鼠相比，正常治疗组无显著差异。通过组织病理学检查证实其抗氧化特性，从而对stz诱导的糖尿病大鼠具有保护作用。本研究结果表明，d-柠檬烯除具有抗糖尿病作用外，还具有抗氧化作用。
The aim of this study was to evaluate the protective effects of d-limonene on the levels of lipid peroxidation by-products and antioxidant defence systems in the plasma and tissues of normal and streptozotocin (STZ)-induced diabetes rats. The experimental diabetes was induced in rats by a single dose of STZ (40mg/kg i.p.) injection, and treatment with d-limonene was continued for 45days. After the treatment period, oxidative stress parameters such as lipid peroxidation by-products; enzymatic antioxidants such as superoxide dismutase, catalase, glutathione peroxidase and glutathione-S-transferase; non-enzymic antioxidants including reduced glutathione, Vitamins C and E were measured in the plasma and tissues of experimental rats. An increase in the levels of lipid peroxidation by-products and significant decrease in antioxidant enzymes were observed in untreated diabetic rats. Administration of d-limonene to diabetic rats for 45days caused a significant reduction in the levels of lipid peroxidation by-products and an increase in the activities of antioxidant enzymes, when compared with the untreated diabetic group. There was no significant difference in normal treated groups, when compared with normal rats. Biochemical observations were substantiated with the help of histopathological examinations through its antioxidant properties and thereby conferred protection against STZ-induced diabetic rats. The result of this study indicates that d-limonene has antioxidant potential in addition to its antidiabetic effect in experimental diabetes.