Teflon Spray Ionization Mass Spectrometry
Teflon Spray Ionization Mass Spectrometry
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DOI：
10.1021/jasms.9b00069
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发表时间：
2020-02-01
影响因子：
3.2
通讯作者：
Zare, Richard N.
中科院分区：
文献类型：
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作者：
Narayanan, Rahul;Song, Xiaowei;Zare, Richard N.
聚四氟乙烯，通常称为特氟龙，是一种以其惰性，强度和不粘特性而闻名的塑料，允许其在许多应用中重复使用。我们报告使用三角形切割的聚四氟乙烯基板，以取代纸的形式喷雾质谱。高电位（正或负）下的导线（金）与三角形顶点处的液体样品液滴接触，导致液滴喷雾指向质谱仪的加热入口。类固醇、药物、非法添加剂、肽、蛋白质、胆红素和万古霉素的质谱具有高信噪比（S/N），允许在纳克/毫升（ng/mL）水平进行检测。对这些分析物中的每一种的检查表明，在相同条件下，特氟隆喷雾比纸喷雾更敏感几个数量级。特氟隆喷雾电离质谱法应用于生物流体样品的代谢组学和脂质组学分析。使用浓度为10 μ g/mL的Teflon喷雾实现多环芳烃的检测。这些实验表明，在检测许多环境和生物相关的系统，具有高灵敏度和S/N比使用聚四氟乙烯在正常的纸基板的优势。
Polytetrafluoroethene, commonly known as Teflon, is a plastic famous for its inertness, strength, and nonstick properties, allowing its repeated use in many applications. We report the use of a triangularly cut Teflon substrate to take the place of paper in a form of spray mass spectrometry. A conducting wire (gold) at high potential (positive or negative) makes contact with a drop of the liquid sample at the apex of the triangle, causing a spray of droplets to be directed toward the heated inlet of a mass spectrometer. Saccharides, drugs, illegal additives, peptides, proteins, bilirubin, and vancomycin give mass spectra with high signal-to-noise (S/N) ratios, allowing detection at the nanogram per milliliter (ng/mL) level. Examination of each of these analytes demonstrates that Teflon spray is several orders of magnitude more sensitive than paper spray under the same conditions. Teflon spray ionization mass spectrometry is applied to the metabolomic and lipidomic profiling of biological fluid samples. Detection of polycyclic aromatic hydrocarbons is achieved with Teflon spray at 10 mu g/mL concentrations. These experiments show the advantage of using Teflon over a normal paper substrate in detecting many environmentally and biologically relevant systems with high sensitivity and S/N ratio.