Genomic features of attenuated Junin virus vaccine strain candidate
Genomic features of attenuated Junin virus vaccine strain candidate
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DOI：
10.1007/s11262-005-5843-2
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发表时间：
2006-02-01
期刊：
影响因子：
1.6
通讯作者：
Lozano, ME
中科院分区：
文献类型：
--
作者：
Goñi, SE;Iserte, JA;Lozano, ME
朱宁病毒株Candid#1是作为阿根廷出血热减毒活疫苗开发的。本文报道了Candid#1的L RNA的核苷酸序列，并研究了Candid#1与其更强毒力的祖先朱宁病毒XJ#44和XJ13（原型）以及其他近缘和远缘沙粒病毒的关系。Candid#1及其祖先菌株的L和Z基因的核苷酸和氨基酸序列的比较揭示了疫苗菌株特有的L多肽中的12个点突变。这些变化可能暂时与减毒表型相关。相反，Z ORF在所有菌株中完全保守。
Junin virus strain Candid #1 was developed as a live attenuated vaccine for Argentine haemorrhagic fever. In this paper, we report the nucleotide sequences of L RNA of Candid #1 and examine the relationship to its more virulent ancestors Junin virus XJ#44 and XJ 13 (prototype) and other closely and distantly related arenaviruses. Comparisons of the nucleotide and amino acid sequences of L and Z genes of Candid #1 and its progenitor strains revealed twelve point mutations in the L polypeptide that are unique to the vaccine strain. These changes could be provisionally associated with the attenuated phenotype. In contrast, Z ORF was completely conserved among all strains.