Novel Application of Red Fluorescent Protein (DsRed-Express) for the Study of Functional Dynamics of Nuclear Receptors
Novel Application of Red Fluorescent Protein (DsRed-Express) for the Study of Functional Dynamics of Nuclear Receptors
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DOI：
10.1007/s10895-017-2109-z
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发表时间：
2017-07-01
影响因子：
2.7
通讯作者：
Tyagi, Rakesh K.
中科院分区：
文献类型：
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作者：
Dash, Amit K.;Yende, Ashutosh S.;Tyagi, Rakesh K.
荧光蛋白的到来彻底改变了我们在分子细胞生物学领域的研究方式。在本研究中，我们已经成功地利用了在活细胞中的红色荧光蛋白（RFP）的多聚体形成的属性，优先转移的unliganded核受体从核到胞质隔室。随后，这些胞质移位的非配体受体可以被其配体诱导移位到细胞核中。虽然RFP作为蛋白质标签的多聚化被视为缺点，但我们已经开发并预测了这种特性在验证临床药物，草药化合物，这种胞质转移的转录因子可以提供一个独特的机会，通过分析配体-受体相互作用和功能动力学来研究受体-配体相互作用。介导的受体从细胞质区室转移到活细胞的细胞核。
Arrivals of fluorescent proteins have revolutionized the way we do research in the areas of molecular cell biology. In the present study, we have successfully exploited the multimer-forming property of Red Fluorescent Protein (RFP) in living cells to preferentially shift the unliganded nuclear receptors from the nuclear to the cytoplasmic compartment. Subsequently, these cytoplasmic-shifted unliganded receptors could be induced to translocate into the nucleus by their ligands. Though the multimerization of RFP as a protein-tag is viewed as a disadvantage, we have exploited and projected this property towards novel applications in validating the clinical drugs, herbal compounds, metabolic disruptors etc. Such cytoplasmic shifted transcription factors can offer a unique opportunity to study receptor-ligand interactions and functional dynamics by analyzing ligand-mediated receptor translocation from cytoplasmic compartment to the nucleus of living cells.