Infection of grass carp reovirus induced the expressional suppression of pro-viral Fibulin-4 in host cells
Infection of grass carp reovirus induced the expressional suppression of pro-viral Fibulin-4 in host cells
复制标题
草鱼呼肠孤病毒感染导致宿主细胞中前病毒Fibulin-4表达抑制
DOI：
10.1016/j.fsi.2018.04.014
复制
发表时间：
2018
影响因子：
4.7
通讯作者：
Lu Liqun
中科院分区：
文献类型：
--
作者：
Sheng Jialu;Yu Fei;Chen Dubo;Wang Hao;Lu Liqun
纤维蛋白-4不仅参与结缔组织的发育和弹性纤维的形成，而且通过激活Wnt/β-catenin信号通路在肿瘤生长中发挥重要作用。最近发现FiBulin-4与草鱼呼肠孤病毒(GCRV)外衣壳蛋白有关，可能与草鱼病毒性出血性疾病有关。在这里，我们从翻译和转录水平上监测了GCRV感染过程中Fiblin-4的表达模式，发现Fiblin-4在草帽GCO细胞中对病毒攻击具有显著的抑制作用。Western印迹和实时荧光定量RT-PCR分析证实，将pEGFP-Fiblin-4载体导入GCO细胞后，可获得高表达的纤维蛋白-4。在纤维蛋白-4过表达的GCO细胞中，病毒蛋白合成和子代病毒产量显著增加。我们的研究表明，FiBulin-4具有促病毒功能，并在病毒攻击过程中受到抑制。因此，抑制纤维蛋白-4的表达似乎与草鱼的抗病毒反应有关。
Fibulin-4 is not only involved in connective tissue development and elastic fiber formation, but also plays critical neoplastic roles in tumor growth by activating Wnt/β-Catenin signaling in human. Recently, Fibulin-4 was shown to associate with grass carp reovirus (GCRV) outer capsid proteins and might relate to viral hemorrhagic disease in grass carpCtenopharyngodon idella. Here, we monitored the expression pattern of Fibulin-4 during the infection course of GCRV at both translational and transcriptional levels, and found that Fibulin-4 was significantly suppressed upon the viral challenge in grass cap GCO cells. Over expression of Fibulin-4 was achieved by transduction of pEGFP-Fibulin-4 plasmids into GCO cells, which was confirmed by both Western blot and Real time RT-PCR analysis. In GCO cells with over-expression of Fibulin-4, significantly increase of viral protein synthesis and progeny virus production was detected. Our study indicated that Fibulin-4 displayed pro-viral function and was inhibited during viral challenge. Thus, repression of Fibulin-4 expression seemed to be involved in anti-viral response in grass carpCtenopharyngodon idella.