HEAT-SHOCK INDUCES CHROMOSOME LOSS IN THE YEAST CANDIDA-ALBICANS
HEAT-SHOCK INDUCES CHROMOSOME LOSS IN THE YEAST CANDIDA-ALBICANS
复制标题
DOI：
10.1007/bf00383330
复制
发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
POULTER, R
中科院分区：
文献类型：
--
作者：
HILTON, C;MARKIE, D;POULTER, R
本文所述的热休克方案导致有丝分裂在对数C期不稳定。白色念珠菌细胞这种不稳定性在二倍体、非整倍体和四倍体品系中诱导。所分析的菌株是多个杂合子，这有助于检测由纯合子形成所表现的有丝分裂不稳定性。先前显示携带顺式连锁突变等位基因的菌株显示扭结等位基因的一致分离。相反，携带非连锁突变等位基因的菌株则不显示这种一致分离。这种完全连锁群的分离表明，热激诱导C。白色念珠菌该方法在半知菌C.白色念珠菌至少有3条染色体。
The heat shock protocol described in this paper causes mitotic instability in log phase C. albicans cells. Such instability in induced in diploid, aneuploid and tetraploid strains. The strains analyzed are multiple heterozygotes which facilitates the detection of mitotic instability as manifested by the formation of homozygotes. Strains previously shown to be carrying cis linked mutant alleles show coincident segregation of the kinked alleles. Conversely, strains which carry unlinked mutant alleles display no such coincident segregation. This segregation of complete linkage groups suggests that heat shock is inducing chromosome loss in C. albicans. The application of this protocol to the genetics of the imperfect fungus C. albicans has produced evidence of at least 3 chromosomes.