Metabolic and functional profiling of the ischemic/reperfused rat retina
Metabolic and functional profiling of the ischemic/reperfused rat retina
复制标题
缺血/再灌注大鼠视网膜的代谢和功能分析
DOI：
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复制
发表时间：
2007
期刊：
影响因子：
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通讯作者：
M. Kalloniatis
中科院分区：
文献类型：
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作者：
Daniel Sun;A. Vingrys;M. Kalloniatis
我们定量跟踪了缺血性损伤后长达两周的不同视网膜元件内氨基酸标记和阳离子通道功能的恢复情况。使用多种氨基酸和胍丁胺（阳离子通道探针；1-氨基-4-胍基丁烷；AGB）免疫细胞化学模式的模式识别分析来对视网膜内的所有神经元件进行分类。这种分类在空间上是完整的并且具有单细胞分辨率。到再灌注 48 小时时，几乎所有细胞群的氨基酸标记模式都恢复到接近缺血前的水平，但谷氨酰胺和丙氨酸水平除外，这两种细胞群在许多细胞群中仍然显着较高。分类导致总共 18 个统计上可分离的主题类别（包括神经元、神经胶质细胞和视网膜外类别），比正常视网膜减少了 10 个主题类别（Sun 等人 [2007a, b] J Comp Neurol，本期）。除了内核层内已知的无长突细胞类型的选择性丢失之外，我们现在还证明了双极细胞群中代表视锥双极细胞的主题类别的选择性丢失。虽然氨基酸标记模式有所恢复，但在几个主题类别中存在持续的阳离子通道门控异常（如 AGB 标记所反映），包括代表所有剩余杆状双极细胞的主题类别，表明继发于代谢损伤的异常神经元功能。 J.Comp。内罗尔。 505:114–130, 2007。© 2007 Wiley-Liss, Inc.
We quantitatively tracked the recovery in amino acid labeling and cation channel functionality within distinct retinal elements for up to 2 weeks after an ischemic insult. Pattern recognition analysis of multiple amino acid and agmatine (a cation channel probe; 1‐amino‐4‐guanidobutane; AGB) immunocytochemical patterns was used to classify all neural elements within the retina. This classification was spatially complete and with single‐cell resolution. By 48 hours of reperfusion the amino acid labeling pattern of virtually all cell populations had returned to near preischemic levels, with the exception of glutamine and alanine levels, which remained significantly higher in many cell populations. Classification resulted in a total of 18 statistically separable theme classes (including neurons, glia, and extraretinal classes), a reduction of 10 theme classes from the normal retina (Sun et al. [ 2007a , b ] J Comp Neurol, this issue). In addition to the known selective losses of amacrine cell types within the inner nuclear layer, we now demonstrate a selective loss of theme classes representing cone bipolar cells within the bipolar cell population. While there was a recovery in the amino acid labeling pattern, there were persistent cation channel gating anomalies (as reflected by AGB labeling) within several theme classes, including the theme class representing all the remaining rod bipolar cells, suggesting aberrant neuronal function secondary to metabolic insult. J. Comp. Neurol. 505:114–130, 2007. © 2007 Wiley‐Liss, Inc.
分离的猫视网膜中的氨基酸特征。
DOI：
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发表时间：
1998
期刊：
Investigative ophthalmology & visual science.
影响因子：
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作者：
Marc,RE;Murry,RF;Fisher,SK;Linberg,KA;Lewis,GP
通讯作者：
Lewis,GP
影响因子：
5.1
作者：
Gross, RL;Hensley, SH;Wu, SM
通讯作者：
Wu, SM
广谱半胱天冬酶抑制剂可挽救缺血后的视网膜神经节细胞。
影响因子：
1.7
作者：
Patil,Kiran;Sharma,SansarC
通讯作者：
Sharma,SansarC