Designing ordered micropatterned hydroxyapatite bioceramics to promote the growth and osteogenic differentiation of bone marrow stromal cells
Designing ordered micropatterned hydroxyapatite bioceramics to promote the growth and osteogenic differentiation of bone marrow stromal cells
复制标题
设计有序微图案羟基磷灰石生物陶瓷促进骨髓基质细胞的生长和成骨分化
DOI：
10.1039/c4tb01838a
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发表时间：
2015-01-01
影响因子：
7
通讯作者：
Lin, Kaili
中科院分区：
文献类型：
--
作者：
Zhao, Cancan;Xia, Lunguo;Lin, Kaili
图案化的结构化表面对于控制细胞的微环境和调节某些细胞反应（例如细胞粘附、迁移、增殖和分化）非常有用。本文以有序微图案化尼龙筛为模板，在羟基磷灰石[Ca-10（PO 4）（6）（OH）（2），HAp]生物陶瓷表面制备了由不同尺寸的方形凸起构成的有序微图案。通过对模板网格的简单裁剪，可以方便地调节图案凸起的高度、宽度和间距。与传统的平面材料相比，微图案化HAp生物陶瓷具有更好的润湿性和更高的表面能，显著促进了大鼠骨髓基质细胞（bMSCs）的黏附、增殖和成骨分化。此外，与较大的图案尺寸相比，使用接近于细胞尺寸的图案尺寸显示出更好的细胞反应刺激。我们的研究表明，微图案化结构的HAp生物陶瓷的制造是至关重要的骨再生和细胞培养基质应用设计最佳的生物材料。
Patterned structured surfaces are very useful to control a cell's microenvironment and to modulate certain cellular responses, such as cell adhesion, migration, proliferation, and differentiation. Herein, ordered micropatterns constructed by a quadrate convex with different sizes were fabricated on a hydroxyapatite [Ca-10(PO4)(6)(OH)(2), HAp] bioceramic surface using an ordered micropatterned nylon sieve as templates. The height, width and space of the convex for the patterns could be facilely regulated via simply tailoring the meshes of the template. Compared with traditional samples with flat surfaces, the fabricated HAp bioceramics with micropatterned surfaces possessed better wettability and higher surface energy, which significantly promoted the adhesion, proliferation, and osteogenic differentiation of rat bone marrow stromal cells (bMSCs). Furthermore, using a pattern size close to that of the cell size showed a better stimulation of cell response compared with larger pattern sizes. Our study suggests that the fabrication of micropatterned structured HAp bioceramics is critical for designing optimal biomaterials for bone regeneration and cell culture substrate applications.