REDUCED NA+-K+-ATPASE ACTIVITY AND PLASMA LYSOPHOSPHATIDYLCHOLINE CONCENTRATIONS IN DIABETIC-PATIENTS
REDUCED NA+-K+-ATPASE ACTIVITY AND PLASMA LYSOPHOSPHATIDYLCHOLINE CONCENTRATIONS IN DIABETIC-PATIENTS
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DOI：
10.2337/diabetes.43.7.915
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发表时间：
1994-07-01
期刊：
影响因子：
7.7
通讯作者：
MAZZANTI, L
中科院分区：
文献类型：
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作者：
RABINI, RA;GALASSI, R;MAZZANTI, L
正常人血浆中抑制Na+-K+ atp酶的部分最近被鉴定为溶血磷脂酰胆碱（LPC）。本研究旨在探讨糖尿病患者细胞膜Na+-K+- atp酶活性与血浆LPC之间的关系。我们研究了10例胰岛素依赖型糖尿病（IDDM）患者，14例非胰岛素依赖型糖尿病（NIDDM）患者和10例性别和年龄相匹配的对照组。与对照组相比，IDDM和NIDDM患者血浆LPC浓度均升高。两组患者红细胞和血小板膜Na+-K+- atp酶活性均降低。血浆LPC浓度与红细胞和血小板膜N2(+)-K+- atp酶活性呈极显著相关（P < 0.01）。为了研究LPC对Na+-K+- atp酶的影响，我们测定了6名健康受试者在LPC浓度（1-10 μ M）的体外培养后红细胞膜上Na+-K+- atp酶的活性。体外LPC浓度为2.5 μ M时，酶活性显著降低，浓度为5 μ M时，酶活性进一步降低。这些数据表明，糖尿病患者Na+-K+- atp酶活性的降低可能与LPC浓度升高有关。
A fraction from normal human plasma inhibiting Na+-K+ ATPase has been recently identified as lysophosphatidylcholine (LPC). The aim of this study was to investigate the existence of a relationship between the activity of the cellular membrane Na+-K+-ATPase and plasma LPC in human diabetes. We studied 10 patients with insulin-dependent diabetes mellitus (IDDM), 14 patients with non-insulin-dependent diabetes mellitus (NIDDM), and 10 sex- and age-matched control subjects. Plasma LPC concentrations were increased in both IDDM and NIDDM patients compared with control subjects. Na+-K+-ATPase activity was reduced in both groups of patients in erythrocyte and platelet membranes. There was a significant correlation between the concentrations of plasma LPC and N2(+)-K+-ATPase activity in both erythrocyte and platelet membranes (P < 0.01). To investigate the effect of LPC on the enzyme, Na+-K+-ATPase activity was determined in erythrocyte membranes obtained from six healthy subjects after in vitro incubation with increasing concentrations of LPC (1-10 mu M). Enzymatic activity was significantly reduced by in vitro LPC at a concentration of 2.5 mu M, with a further decrease at 5 mu M. These data suggest that the decrease in Na+-K+-ATPase activity in diabetes might be due to increased LPC concentrations.