Identification of the gene loci that predispose to rheumatoid arthritis
Identification of the gene loci that predispose to rheumatoid arthritis
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DOI：
10.1093/intimm/10.12.1891
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发表时间：
1998-12-01
影响因子：
4.4
通讯作者：
Imura, S
中科院分区：
文献类型：
--
作者：
Shiozawa, S;Hayashi, S;Imura, S
我们使用基于荧光的微卫星标记分析和受累同胞对连锁研究在人类基因组中寻找易患类风湿性关节炎（RA）的基因。一组41个日本家庭，每个家庭至少有两个受累同胞，对全基因组358个多态性微卫星标记位点进行分型。用荧光标记的引物进行PCR扩增，根据DNA上CA重复序列的差异大小，用最大lod得分（MLS）进行连锁分析，D1 S214和D8 S556的MLS分别为3.27和3.33，而HLA-DRB 1区域的MLS为3.33。
We have searched the human genome for genes that predispose to rheumatoid arthritis (RA) using fluorescence-based microsatellite marker analysis and affected sib-pair linkage study, A panel of 41 Japanese families, each with at least two affected siblings, was typed for genome-wide 358 polymorphic microsatellite marker loci. Markers were amplified by the PCR using fluorescence-tagged primers and sized based on the difference of CA repeats on DNA, Linkage analysis was made using maximum lod score (MLS), The MLS for D1S214 and D8S556 was 3.27 and 3.33, while the MLS for the HLA-DRB1 region was