INHIBITION OF THE MG(II).ATP-DEPENDENT PHOSPHOPROTEIN PHOSPHATASE BY THE REGULATORY SUBUNIT OF CAMP-DEPENDENT PROTEIN-KINASE
INHIBITION OF THE MG(II).ATP-DEPENDENT PHOSPHOPROTEIN PHOSPHATASE BY THE REGULATORY SUBUNIT OF CAMP-DEPENDENT PROTEIN-KINASE
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DOI：
10.1073/pnas.82.22.7565
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
MERLEVEDE, W
中科院分区：
文献类型：
--
作者：
JURGENSEN, SR;CHOCK, PB;MERLEVEDE, W
We report potent inhibition of the Mg(II).cntdot.ATP-dependent protein phosphatase, FC.cntdot.M, by the regulatory subunit dimer of type II cAMP-dependent protein kinase, R2II. The protein kinase catalytic subunit has no effect on phosphatase activity and is unable to substitute for kinase FA in the kinase FA- and Mg(II).cntdot.ATP-mediated phosphatase activation reaction. Phosphatase inhibition was investigated as a function of R2II concentration. The results suggest that R2II both inhibits the active phosphatase and inhibits phosphatase activation. The inhibition is shown to be noncompetitive with respect to substrate (phosphorylase a). The potential physiological significance of this inhibition is discussed in terms of phosphorylation/dephosphorylation cascade systems involving this kinase and phosphatase.
