Cellular Force Measurement Using a Nanometric-Probe-Integrated Microfluidic Chip with a Displacement Reduction Mechanism
Cellular Force Measurement Using a Nanometric-Probe-Integrated Microfluidic Chip with a Displacement Reduction Mechanism
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DOI：
10.20965/jrm.2013.p0277
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发表时间：
2013-04-01
影响因子：
1.1
通讯作者：
Arai, Fumihito
中科院分区：
文献类型：
--
作者：
Sakuma, Shinya;Arai, Fumihito
提出了一种微流控芯片中高输出力探针针尖的非接触纳米定位方法。为了测量细胞变形的基础上，在微流控芯片中的细胞力，我们采用了一个芯片上的探针与磁驱动方法与驱动的毫牛顿的顺序。提出了一种用于实现探针针尖定位的纳米级分辨率的减小机构。该机构利用具有不同刚度水平的弹簧的组合，并由磁力驱动。原型装置的性能进行了检查，结果表明，作为重复定位精度的措施，探针尖端位移的标准偏差是0.18 μ m以下。成功地测量了变形的卵母细胞的顺序为0.1 mN，证明，作为一个结果，纳米级的非接触式驱动的芯片上的探针具有高输出力。利用该探针实现了微流控芯片细胞力的测量。
This paper presents noncontact nanometric positioning of a probe tip with high output force in a microfluidic chip. To measure cellular force in a microfluidic chip on the basis of cell deformation, we employed an on-chip probe with a magnetic drive method with actuation on the order of millinewtons. A reduction mechanism was proposed to realize nanometric resolution for positioning the probe tip. This mechanism utilizes a combination of springs with different stiffness levels and is driven by magnetic force. The performance of the prototype device was examined and results indicated that, as a measure of repetitive positioning accuracy, standard deviation of probe tip displacement was under 0.18 mu m. Deformation was successfully measured for an oocyte on the order of 0.1 mN, demonstrating, as a consequence, nanometric order noncontact actuation of the on-chip probe with high output force. Using this on-chip probe, cellular force measurement was achieved for the microfluidic chip.