Transmission of hepatitis C by intrahepatic inoculation with transcribed RNA
Transmission of hepatitis C by intrahepatic inoculation with transcribed RNA
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DOI：
10.1126/science.277.5325.570
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发表时间：
1997-07-25
期刊：
影响因子：
56.9
通讯作者：
Rice, CM
中科院分区：
文献类型：
--
作者：
Kolykhalov, AA;Agapov, EV;Rice, CM
世界上超过1%的人口慢性感染丙型肝炎病毒（HCV）。HCV感染可导致急性肝炎、慢性肝炎和肝硬化，这与肝细胞癌的发展密切相关。由于缺乏真正的传染性分子克隆，HCV复制的遗传学研究一直受到阻碍。构建了HCV互补DNA的全长功能克隆。克隆的RNA转录本被发现具有传染性，并在黑猩猩直接肝内接种后引起疾病。这项工作定义了功能性HCV基因组RNA的结构，并证明HCV本身足以引起疾病。
More than 1% of the world's population is chronically infected with hepatitis C virus (HCV). HCV infection can result in acute hepatitis, chronic hepatitis, and cirrhosis, which is strongly associated with development of hepatocellular carcinoma. Genetic studies of HCV replication have been hampered by lack of a bona fide infectious molecular clone. Full-length functional clones of HCV complementary DNA were constructed. RNA transcripts from the clones were found to be infectious and to cause disease in chimpanzees after direct intrahepatic inoculation. This work defines the structure of a functional HCV genome RNA and proves that HCV alone is sufficient to cause disease.