Purification and characterization of prolixin S (nitrophorin 2), the salivary anticoagulant of the blood-sucking bug Rhodnius prolixus.
Purification and characterization of prolixin S (nitrophorin 2), the salivary anticoagulant of the blood-sucking bug Rhodnius prolixus.
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Prolixin S（硝基蛋白 2）的纯化和表征，Prolixin S 是吸血虫 Rhodnius prolixus 的唾液抗凝剂。
DOI：
10.1042/bj3080243
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Guimarães,JA
中科院分区：
文献类型：
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作者：
Ribeiro,JM;Schneider,M;Guimarães,JA
采用弱阳离子交换层析、DEAE层析、疏水层析和十八烷基反相层析等方法，对长须红蝽（Rhodnius prolixus）的唾液抗凝剂进行了纯化，得到了一个与其唾液腺中的四种NO血红素蛋白载体之一的硝基蛋白2（nitrophorin 2）具有相同N端序列的蛋白质，其分子量为19689 Da [D.香槟，RH Nussenzveig和J.M.C. Ribeiro，（1995）J.Biol.Chem.270，出版中]。为了排除硝化蛋白是污染物的可能性，进行了另一种色谱方案，包括色谱聚焦，然后是强阳离子交换色谱。再一次，用硝化蛋白2洗脱抗凝剂。Nitrophorin 2抑制凝血因子VIII介导的因子X活化，并解释了在Rhodnius唾液腺中观察到的所有抗凝血活性。
The salivary anticoagulant of the blood-sucking bug Rhodnius prolixus was purified to homogeneity using a protocol consisting of weak cation-exchange, DEAE, hydrophobic-interaction and octadecyl reverse-phase chromatography, yielding a protein with the same N-terminal sequence as nitrophorin 2, one of the four NO haem protein carriers present in the salivary glands of Rhodnius with a molecular mass of 19689 Da [D. Champagne, R.H. Nussenzveig and J.M.C. Ribeiro, (1995) J. Biol. Chem. 270, in the press]. To exclude the possibility of the nitrophorin being a contaminant, another chromatographic protocol was performed, consisting of chromatofocusing followed by strong-cation-exchange chromatography. Again the anticoagulant was eluted with nitrophorin 2. Nitrophorin 2 inhibits coagulation Factor VIII-mediated activation of Factor X and accounts for all the anti-clotting activity observed in Rhodnius salivary glands.