SOMATIC EMBRYOGENESIS AND POLYEMBRYOGENESIS IN DOUGLAS-FIR CELL-SUSPENSION CULTURES
SOMATIC EMBRYOGENESIS AND POLYEMBRYOGENESIS IN DOUGLAS-FIR CELL-SUSPENSION CULTURES
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DOI：
10.1016/0168-9452(87)90056-2
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发表时间：
1987-01-01
期刊：
影响因子：
5.2
通讯作者：
GUPTA, PK
中科院分区：
文献类型：
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作者：
DURZAN, DJ;GUPTA, PK
从道格拉斯杉木（pseudosuga menziesiii, Mirb.）的未成熟胚中形成增殖胚柄团（ESM）。Franco)，受精后4-5周，在含有2,4-二氯苯氧乙酸（2,4- d）、动蛋白和n6 -苄基氨基嘌呤（BAP）的改良MS培养基上。esm在含有低2,4- d、动蛋白和BAP的改性DCR培养基上作为细胞悬浮培养维持6个月以上。在悬浮培养中，随着植物生长调节剂的逐渐减少和脱落酸的添加，单个体细胞胚的发育开始了。道格拉斯杉的合子胚胎发生的早期阶段在针叶树中是独特的，劈裂多胚胎发生是未知的。在体细胞胚胎发生中，通过用脱落酸抑制卵裂多胚胎发育和不使用生长调节剂培养单个胚胎，再现了合子胚胎发育的特征阶段，恢复了完整胚胎。组织学检查证实体细胞胚胎双极性组织。当转化率较低时，具有多个子叶的植株已转移到土壤中并继续生长并产生新芽。
A proliferating embryonal-suspensor mass (ESM) was initiated from immature embryos of Douglas-fir (Pseudotsuga menziesiii (Mirb.) Franco), 4-5 weeks after fertilization, on modified MS medium with 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin, and N6-benzylaminopurine (BAP). ESMs were maintained for over 6 months as cell suspension cultures on modified DCR media with low 2,4-D and with kinetin and BAP. The development of individual somatic embryos was initiated in suspension culture by the gradual reduction of plant growth regulators and by addition of abscisic acid. The early stages of zygotic embryogenesis in Douglas-fir are unique among conifers and cleavage polyembryogenesis is unknown. In somatic embryogenesis, characteristic stages of zygotic embryonic development were recapitulated and complete embryos were recovered by inhibiting cleavage polyembryony with abscisic acid and culturing individual embryos without growth regulators. Histological examination confirmed bipolar organization of somatic embryos. While conversion is low, plantlets with multiple cotyledons have been transferred to soil and continue to grow with production of a new shoot.