Signals that release growth factors from renal epithelial cells.
Signals that release growth factors from renal epithelial cells.
复制标题
从肾上皮细胞释放生长因子的信号。
DOI：
10.1016/s0272-6386(12)80335-0
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Kartha,S
中科院分区：
文献类型：
--
作者：
Toback,FG;Walsh-Reitz,MM;Kartha,S
非转化BSC-1系的猴肾上皮细胞已用作研究生长控制的模型系统。通过降低培养基中的钾浓度，加速细胞增殖，在培养中研究了钾耗竭性肾病的肾脏生长。这种反应似乎是通过释放表观分子量为12，000至30，000的促生长活性来介导的。当培养基的Na浓度降低时也观察到生长刺激，并且与表现出细胞类型特异性的两种生长促进因子（表观分子量，6，200和9，000）的出现相关。因此，细胞外K或Na浓度的适度降低导致自分泌因子的快速出现，这些自分泌因子可沿肾单位沿着调节细胞功能。BSC-1细胞最强的促分裂剂是二磷酸腺苷（ADP）。这种核苷酸刺激几种细胞周期特异性基因和原癌基因的表达，并诱导对BSC-1细胞无促有丝分裂作用的血小板衍生生长因子样蛋白的分泌。肾上皮细胞在体内释放这种生长因子将代表启动邻近基质或血管细胞增殖的旁分泌机制。
Monkey kidney epithelial cells of the nontransformed BSC-1 line have been used as a model system to investigate growth control. Renal growth in K depletion nephropathy was studied in culture by reducing the K concentration of the medium, which accelerated cell proliferation. This response appeared to be mediated by release of a growth-promoting activity that has an apparent molecular weight of 12,000 to 30,000. Growth stimulation was also observed when the Na concentration of the medium was reduced and was associated with the appearance of two growth-promoting factors (apparent molecular weight, 6,200 and 9,000) that exhibited cell-type specificity. Thus, modest reductions in the extracellular concentration of K or Na result in rapid appearance of autocrine factors that could modulate cell function along the nephron. The most powerful mitogen for BSC-1 cells is adenosine diphosphate (ADP). This nucleotide stimulates expression of several cell cycle-specific genes and proto-oncogenes, and induces secretion of a platelet-derived growth factor-like protein that is not mitogenic for BSC-1 cells. Release of this growth factor by renal epithelial cells in vivo would represent a paracrine mechanism to initiate proliferation of neighboring stromal or vascular cells.