The analysis of large-scale gene expression correlated to the phase changes of the migratory locust
The analysis of large-scale gene expression correlated to the phase changes of the migratory locust
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DOI：
10.1073/pnas.0407753101
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发表时间：
2004-12-21
影响因子：
11.1
通讯作者：
Yu, J
中科院分区：
文献类型：
--
作者：
Kang, L;Chen, XY;Yu, J
飞蝗是最臭名昭著的农业害虫之一，它经历了一个众所周知的可逆的、依赖密度的从独居到群居的相变过程。为了阐明这种相变的潜在分子机制，我们在两个阶段分别从全身和解剖器官中产生了76,012个EST。比较12,161个Unigene簇，我们确定了532个基因是与阶段相关的(P<；0.01)。对时相相关表达的综合评估表明，虽然从后腿和中肠的不同类别中的大多数基因在群居阶段下调，但头部的几个基因类别显著上调，包括具有肽酶、受体和氧结合活性的基因，以及与发育、细胞生长和对外部刺激的反应有关的基因。其中，包括保幼激素结合蛋白、六角体蛋白、酚氧化酶原和血蓝蛋白在内的JHPH超家族蛋白在群居型棉铃虫的头部和独生型棉铃虫的后腿中高表达。定量聚合酶链式反应实验部分证实了EST结果。这些差异调控的基因具有很强的功能暗示，即许多分子活动参与了阶段可塑性。这项研究为半代谢昆虫提供了丰富的分子标记和基因组信息，并深入了解了蝗虫相变的遗传和分子机制。
The migratory locust is one of the most notorious agricultural pests that undergo a well known reversible, density-dependent phase transition from the solitary to the gregarious. To demonstrate the underlying molecular mechanisms of the phase change, we generated 76,012 ESTs from the whole body and dissected organs in the two phases. Comparing 12,161 unigene clusters, we identified 532 genes as phase-related (P < 0.01). Comprehensive assessment of the phase-related expression revealed that, whereas most of the genes in various categories from hind legs and the midgut are down-regulated in the gregarious phase, several gene classes in the head are impressively up-regulated, including those with peptidase, receptor, and oxygen-binding activities and those related to development, cell growth, and responses to external stimuli. Among them, a superfamily of proteins, the JHPH superfamily, which includes juvenile hormone-binding protein, hexamerins, prophenoloxidase, and hemocyanins, were highly expressed in the heads of the gregarious hoppers and hind legs of the solitary hoppers. Quantitative PCR experiments confirmed in part the EST results. These differentially regulated genes have strong functional implications that numerous molecular activities are involved in phase plasticity. This study provides ample molecular markers and genomic information on hemimetabolous insects and insights into the genetic and molecular mechanisms of phase changes in locusts.