Novel versatile smart phone based Microplate readers for on-site diagnoses
Novel versatile smart phone based Microplate readers for on-site diagnoses
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DOI：
10.1016/j.bios.2016.03.049
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发表时间：
2016-07-15
影响因子：
12.6
通讯作者：
Tang, Yong
中科院分区：
文献类型：
--
作者：
Fu, Qiangqiang;Wu, Ze;Tang, Yong
酶标仪是重要的诊断仪器，广泛用于各种读数检测试剂盒（生化分析试剂盒和 ELISA 试剂盒）。然而，由于其昂贵且不便携，商业酶标仪无法用于家庭检测、社区和乡村医院，特别是在发展中国家。在这项研究中，为了提供一种现场便携式、经济高效且多功能的诊断工具，我们报告了一种基于智能手机的新型酶标仪。该装置的基本原理依赖于智能手机的光学传感器来测量液体样品的透射光强度。为了证明这些设备的有效性，开发的基于智能手机的酶标仪被应用于读出各种分析目标的结果。这些目标包括丙氨酸氨基转移酶（ALT；检测限（LOD）为17.54 U/L）、碱性磷酸酶（AKP；LOD为15.56 U/L）、肌酐（LOD为1.35 μM）、牛血清白蛋白（BSA；LOD为0.0041 mg/mL）、前列腺特异性抗原（PSA；LOD为0.76） pg/mL) 和莱克多巴胺 (Rac；LOD 为 0.31 ng/mL)。开发的基于智能手机的酶标仪具有多功能、便携且便宜的特点；他们之所以独一无二，是因为他们有能力在资源和专业知识有限的情况下发挥作用。 (C) 2016 Elsevier B.V. 保留所有权利。
Microplate readers are important diagnostic instruments, used intensively for various readout test kits (biochemical analysis kits and ELISA kits). However, due to their expensive and non-portability, commercial microplate readers are unavailable for home testing, community and rural hospitals, especially in developing countries. In this study, to provide a field-portable, cost-effective and versatile diagnostic tool, we reported a novel smart phone based microplate reader. The basic principle of this devise relies on a smart phone's optical sensor that measures transmitted light intensities of liquid samples. To prove the validity of these devises, developed smart phone based microplate readers were applied to readout results of various analytical targets. These targets included analanine aminotransferase (ALT; limit of detection (LOD) was 17.54 U/L), alkaline phosphatase (AKP; LOD was 15.56 U/L), creatinine (LOD was 1.35 mu M), bovine serum albumin (BSA; LOD was 0.0041 mg/mL), prostate specific antigen (PSA; LOD was 0.76 pg/mL), and ractopamine (Rac; LOD was 0.31 ng/mL). The developed smart phone based microplate readers are versatile, portable, and inexpensive; they are unique because of their ability to perform under circumstances where resources and expertize are limited. (C) 2016 Elsevier B.V. All rights reserved.