EVIDENCE FOR AN ALTERNATIVE PATHWAY OF KERATINOCYTE MATURATION IN PSORIASIS FROM AN ANTIGEN FOUND IN PSORIATIC BUT NOT NORMAL EPIDERMIS
EVIDENCE FOR AN ALTERNATIVE PATHWAY OF KERATINOCYTE MATURATION IN PSORIASIS FROM AN ANTIGEN FOUND IN PSORIATIC BUT NOT NORMAL EPIDERMIS
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DOI：
10.1111/1523-1747.ep12340429
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发表时间：
1984-01-01
影响因子：
6.5
通讯作者：
STREFLING, AM
中科院分区：
文献类型：
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作者：
MANSBRIDGE, JN;KNAPP, AM;STREFLING, AM
一种名为ψ - 3的鼠单克隆抗体被分离出来，它能对银屑病皮肤中成熟的角质形成细胞进行免疫标记，但不能标记正常表皮中的细胞。这种染色是细胞质的，且不能用1%的Triton X - 100提取，这表明ψ - 3抗原是角质形成细胞的一种结构成分。在银屑病皮肤中，基底细胞和浸润的炎症细胞都不被染色，并且该抗原似乎与成熟而非增殖的角质形成细胞特异性相关。从非银屑病个体皮肤体外培养的角质形成细胞在分化细胞中呈颗粒状显示该抗原。在正常皮肤胶带剥离后该抗原也会表达，它代表正常角质形成细胞的一种可诱导产物。该抗原可被蛋白酶K破坏，似乎是一种蛋白质。在不连续十二烷基硫酸钠 - 凝胶电泳中，该抗原的分子量为135,000。ψ - 3抗原被认为是一种在银屑病、细胞培养、伤口愈合以及某些其他皮肤病理状况中表达的新型角质形成细胞产物。如果银屑病中的角质形成细胞成熟是正常系统的一种截断形式，那么就不会预期会合成这样一种新抗原，它支持银屑病角质形成细胞遵循一种替代途径的假说。使用实验性损伤的结果表明，银屑病途径在伤口愈合过程中正常表达。
A murine monoclonal antibody called .psi.-3, which immunolabels maturing keratinocytes in psoriatic skin but not in normal epidermis, was isolated. The staining is cytoplasmic and is not extractable with 1% Triton X-100, which suggests that the .psi.-3 antigen is a structural component of the keratinocyte. Neither basal cells nor invading inflammatory cells are stained in psoriatic skin, and the antigen appears to be associated specifically with maturing and not proliferating keratinocytes. Keratinocytes cultured in vitro from skin from nonpsoriatic individuals display the antigen in a granular pattern in differentiated cells. The antigen is also expressed after tape-stripping of normal skin, and represents an inducible product of normal keratinocytes. The antigen is destroyed by proteinase K, and appears to be a protein. On discontinuous sodium dodecyl sulfate-gel electrophoresis, the antigen has a MW of 135,000. The .psi.-3 antigen is interpreted as a new keratinocyte product expressed in psoriasis, culture, wound healing, and certain other pathologic skin conditions. The synthesis of such a new antigen would not be expected if keratinocyte maturation in psoriasis is a truncated version of the normal system, and it supports the hypothesis that psoriatic keratinocytes are following an alternative pathway. Results using experimental injury suggest that the psoriatic pathway is normally expressed during wound healing.