SENSITIVE ELISA FOR INTERLEUKIN-6 - DETECTION OF IL-6 IN BIOLOGICAL-FLUIDS - SYNOVIAL-FLUIDS AND SERA
SENSITIVE ELISA FOR INTERLEUKIN-6 - DETECTION OF IL-6 IN BIOLOGICAL-FLUIDS - SYNOVIAL-FLUIDS AND SERA
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DOI：
10.1016/0022-1759(91)90063-l
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发表时间：
1991-04-08
影响因子：
2.2
通讯作者：
AARDEN, L
中科院分区：
文献类型：
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作者：
HELLE, M;BOEIJE, L;AARDEN, L
一种抗重组人IL-6的单抗和一种亲和纯化的多克隆抗体已用于ELISA程序以定量检测人IL-6。两种抗体均能中和重组人IL-6和天然人IL-6的生物学活性。以单抗为捕获抗体，以生物素标记的多克隆抗体为检测抗体，结合链霉亲和素辣根过氧化物酶结合物和信号放大系统。对天然IL-6和重组IL-6的检出限均为1pg/ml，与B9生物法检测结果具有较好的相关性，可用于检测类风湿关节炎患者滑液和各种疾病患者血清中的IL-6。IL-6由不同类型的细胞产生，如单核细胞、内皮细胞和平滑肌细胞，或来自生物液，如感染性休克患者的血清或类风湿性关节炎患者的滑液，免疫沉淀在每个病例中只显示分子量在21,000-26,000之间。
A monoclonal antibody and an affinity purified polyclonal antibody, both raised against recombinant human IL-6, have been employed in an ELISA procedure to quantitate human IL-6. Both antibodies were very potent in neutralizing the biological activity of recombinant as well as natural human IL-6. The monoclonal antibody was used as the capture antibody whilst the polyclonal antibody, in biotinylated form, was used as the detecting antibody in combination with a streptavidin horseradish peroxidase conjugate and a signal amplification system. The detection limit for natural as well as recombinant IL-6 was 1 pg/ml. A good correlation was found between the ELISA and the B9 biological assay when IL-6 was measured in crude culture supernatants, in synovial fluids of rheumatoid arthritis patients and in the sera of patients with diverse diseases. Immunoprecipitation of IL-6, produced by different cell types, such as monocytes, endothelial cells and smooth muscle cells or derived from biological fluids, such as the serum of a patient with septic shock or the synovial fluid of a rheumatoid arthritis patient, revealed in every case only molecules in the molecular weight range of 21,000-26,000.