Transcriptome-wide responses of adult melon thrips (Thrips palmi) associated with capsicum chlorosis virus infection
Transcriptome-wide responses of adult melon thrips (Thrips palmi) associated with capsicum chlorosis virus infection
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DOI：
10.1371/journal.pone.0208538
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发表时间：
2018-12-07
期刊：
影响因子：
3.7
通讯作者：
Dietzgen, Ralf G.
中科院分区：
文献类型：
--
作者：
Gamage, Shirani M. K. Widana;Rotenberg, Dorith;Dietzgen, Ralf G.
帕尔米（Thrips palmi）是热带和亚热带地区广泛分布的重要农业害虫，通过取食危害和番茄斑萎病毒的传播给葫芦科和茄科作物造成重大损失。帕尔米（Thrips palmi）是澳大利亚辣椒褪绿病毒（CaCV）的载体。目前对传播生物学的理解和CaCV对T.帕尔米有限。为了深入了解对CaCV感染的分子反应，我们进行了RNA-Seq以鉴定在病毒感染成虫期间差异丰富的蓟马转录物。从T.帕尔米成年人产生了166，445个重叠群，其中约24%包含预测的开放阅读框架。我们确定了1，389个差异表达（DE）转录本，与未暴露的蓟马相比，暴露于病毒的蓟马中上调（708）和下调（681）的数量相当。大约59%的DE转录物与NCBI非冗余蛋白（Blastx）和Blast 2GO识别的病毒暴露的蓟马中上调的转录物中的临时功能类别具有显著匹配，包括先天免疫应答相关基因、唾液腺和/或肠道相关基因和卵黄蛋白原基因。已知大多数免疫相关蛋白在昆虫的溶酶体活性和黑化中起作用。大多数上调的口腔和口腔外消化相关基因似乎参与蛋白质、脂质和植物细胞壁组分的消化，这可能间接提高病毒传播的可能性或频率，或者可能参与调节宿主防御反应。大多数下调的转录本属于基因本体论的“表皮结构成分”的功能范畴。与Frankliniellaocumentalis中对番茄斑萎病毒有反应的DE基因的比较表明，一些蓟马对不同番茄斑萎病毒感染的分子反应是保守的。本研究组装了蓟马属的第一个转录组，为我们拓宽对蓟马和番茄斑萎病毒之间分子相互作用网络的理解提供了重要数据。
Thrips palmi is a widely distributed major agricultural pest in the tropics and subtropics, causing significant losses in cucurbit and solanaceous crops through feeding damage and transmission of tospoviruses. Thrips palmi is a vector of capsicum chlorosis virus (CaCV) in Australia. The present understanding of transmission biology and potential effects of CaCV on T. palmi is limited. To gain insights into molecular responses to CaCV infection, we performed RNA-Seq to identify thrips transcripts that are differentially-abundant during virus infection of adults. De-novo assembly of the transcriptome generated from whole bodies of T. palmi adults generated 166,445 contigs, of which similar to 24% contained a predicted open reading frame. We identified 1,389 differentially-expressed (DE) transcripts, with comparable numbers up- (708) and down-regulated (681) in virus-exposed thrips compared to non-exposed thrips. Approximately 59% of these DE transcripts had significant matches to NCBI non-redundant proteins (Blastx) and Blast2GO identified provisional functional categories among the up-regulated transcripts in virus-exposed thrips including innate immune response-related genes, salivary gland and/or gut-associated genes and vitellogenin genes. The majority of the immune-related proteins are known to serve functions in lysosome activity and melanisation in insects. Most of the up-regulated oral and extra-oral digestion-associated genes appear to be involved in digestion of proteins, lipids and plant cell wall components which may indirectly enhance the likelihood or frequency of virus transmission or may be involved in the regulation of host defence responses. Most of the down-regulated transcripts fell into the gene ontology functional category of 'structural constituent of cuticle'. Comparison to DE genes responsive to tomato spotted wilt virus in Frankliniella occidentalis indicates conservation of some thrips molecular responses to infection by different tospoviruses. This study assembled the first transcriptome in the genus Thrips and provides important data to broaden our understanding of networks of molecular interactions between thrips and tospoviruses.