Improved genetic transformation of the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1
Improved genetic transformation of the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1
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DOI：
10.1093/pcp/pch015
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发表时间：
2004-02-01
影响因子：
4.9
通讯作者：
Ikeuchi, M
中科院分区：
文献类型：
--
作者：
Iwai, M;Katoh, H;Ikeuchi, M
我们改进了电转化和顶层琼脂法相结合的高温蓝细菌，细长热聚球藻BP-1的遗传转化。当使用假定的I型限制性内切核酸酶（tll 2230）的破坏物作为受体细胞时，转化也得到改善。特别地，野生型从未被转化的一些构建体被成功地整合到tll 2230-破坏物中。单交换重组比双交换重组更频繁地被检测到。根据在集胞藻PCC 6803中存在的所有同源基因，我们发现细长集胞藻是天然的外源DNA转化。
We improved genetic transformation of the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1, by combining electroporation with a top agar method. Transformation was also improved when a disruptant of a putative type I restriction endonuclease (tll2230) was used as recipient cells. In particular, some constructs, with which wild type has never been transformed, were successfully integrated into the tll2230-disruptant. Single-crossover recombination was detected more frequently than the double-crossover recombination. In accordance with the presence of all the homologs of pil genes in Synechocystis sp. PCC 6803, we found that T elongatus is naturally transformable with exogenous DNA.