Effects of bilirubin photoisomers on the measurement of direct bilirubin by the bilirubin oxidase method
Effects of bilirubin photoisomers on the measurement of direct bilirubin by the bilirubin oxidase method
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DOI：
10.1177/0004563217716474
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发表时间：
2018-03-01
影响因子：
2.2
通讯作者：
Kusaka, Takashi
中科院分区：
文献类型：
--
作者：
Okada, Hitoshi;Kawada, Kou;Kusaka, Takashi
背景我们偶尔会遇到直接胆红素值增加的再分析过剩的血清收集在过去的新生儿间接高胆红素血症。但这一现象的细节尚不清楚。方法取间接高胆红素血症新生儿的剩余血清，经室内光照24 h，观察血清中直接胆红素的变化及胆红素光异构体之间的关系。采用胆红素氧化酶法（Nescuto和Aqua-autoKainos试剂）和高效液相色谱法测定血清中胆红素含量。暴露前后胆红素光异构体的比值分别为：环胆红素（0.007 ~ 0.29）和（EZ）-胆红素（0.018 ~ 0.041）显著升高，（ZZ）-胆红素显著降低（0.84 ~ 0.55）。结论室内光照后直接胆红素升高，间接高胆红素血症患儿血清中直接胆红素升高与环胆红素升高显著相关。
Background We occasionally encounter increases in direct bilirubin value on reanalysis of the surplus serum collected in the past from a neonate with indirect hyperbilirubinemia. But the details of this phenomenon are unclear. We evaluated the change of direct bilirubin and the relation of bilirubin photoisomer of the serum exposed to room light.Methods Surplus serum samples from neonates with indirect hyperbilirubinemia were exposed to room light for 24h. The bilirubin fraction assay of samples was performed by the bilirubin oxidase method (Nescauto and Aqua-auto Kainos reagent) and high-performance liquid chromatography.Results Direct bilirubin increased significantly from 0.61 to 2.36mg/dL. The respective ratios of bilirubin photoisomers before and after exposure were as follows: cyclobilirubin (0.007 to 0.29) and (EZ)-bilirubin (0.018 to 0.041) increased significantly, (ZZ)-bilirubin decreased 0.84 to 0.55 significantly. The difference of the cyclobilirubin concentration was most closely associated with those of the direct bilirubin concentration.Conclusion Direct bilirubin value was increased after exposure to the room light, and increase in direct bilirubin was significantly correlated by cyclobilirubin increase in the serum samples from neonates with indirect hyperbilirubinemia.