Drugs of abuse in a non-conventional sample; detection of methamphetamine and its main metabolite, amphetamine in abusers' clothes by HPLC with UV and fluorescence detection
Drugs of abuse in a non-conventional sample; detection of methamphetamine and its main metabolite, amphetamine in abusers' clothes by HPLC with UV and fluorescence detection
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DOI：
10.1002/bmc.94
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发表时间：
2001-11-01
影响因子：
1.8
通讯作者：
Nakashima, K
中科院分区：
文献类型：
--
作者：
Al-Dirbashi, OY;Ikeda, K;Nakashima, K
本文报道了已知滥用者服装中甲基苯丙胺及其主要代谢物苯丙胺的检测结果。这些化合物使用氯仿：丙醇（3：1，v/v）的混合物从纺织品中提取，用4-（4，5-二苯基-1H-咪唑-2-基）苯甲酰氯衍生，并使用反相高效液相色谱法分离。通过测量440 nm处的荧光或330 nm处的吸光度来检测衍生物。以1-甲基-3-苯基丙胺为内标，纺织品样品的标准曲线在很宽的范围内呈线性，相关系数为0.997或更好。在信噪比为3的检测限小于或等于37.3和0.4皮克的高效液相色谱-紫外和荧光检测方法，分别在柱上。高浓度和低浓度（n ≥ 3）的日内和日间变化小于或等于12.7%。该方法已成功应用于吸毒者衣服样品中甲基苯丙胺和苯丙胺的检测。版权所有（C）2001约翰威利父子有限公司
In this paper, we report the detection of methamphetamine and its major metabolite, amphetamine, in garments belong to known-abusers. These compounds were extracted from the textile using a mixture of chloroform: propan-2-ol (3:1, v/v), derivatized with 4-(4,5-diphenyl-1H-imidazol-2-yl) benzoyl chloride and separated using a reversed-phase high-performance liquid chromatography. The derivatives were detected by measuring either fluorescence at 440 nm or absorbance at 330 nm. By using 1-methyl-3-phenyl propylamine as an internal standard, calibration curves of spiked textile samples were linear over a wide range with correlation coefficients of 0.997 or better. Detection limits at a signal-to-noise ratio of 3 were less than or equal to 37.3 and 0.4 pg on column for the high-performance liquid chromatography-ultraviolet and -fluorescence detection methods, respectively. Intra- and inter-day variations at high and low concentrations (n greater than or equal to 3) were less than or equal to 12.7%. The developed methods were successfully applied to the determination of methamphetamine and amphetamine in clothes samples belong to abusers. Copyright (C) 2001 John Wiley & Sons, Ltd.