Isolation and characterization of the CYP71D16 trichome-specific promoter from Nicotiana tabacum L.
Isolation and characterization of the CYP71D16 trichome-specific promoter from Nicotiana tabacum L.
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DOI：
10.1093/jxb/erf054
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发表时间：
2002-09
影响因子：
6.9
通讯作者：
E. Wang;S. Gan;G. Wagner
中科院分区：
文献类型：
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作者：
E. Wang;S. Gan;G. Wagner
毛状体是产生分泌物的特化表皮细胞，这些分泌物被认为是抵抗害虫和病原体的第一道防线。许多毛状体分泌的化合物在商业上被用作香料，药物等。这里描述的是烟草毛状体特异性P450基因，CYP 71 D16的启动子的克隆和表征。该启动子被证明是直接的报告基因，β-葡萄糖醛酸酶（GUS），在烟草品种腺毛的特异性表达。T.I.第1068章各方面的发展在完整启动子的情况下，GUS活性主要在腺体细胞中，在与腺体相邻的柄细胞和下部柄细胞中较少。在品种T. I上发现的非腺毛的最远端的毛柄细胞中也观察到GUS染色。1112.启动子缺失分析显示，-223至+111 bp的区域足以指导毛状体特异性表达，但不强烈的腺体表达。对文献的研究表明，这是第一个特征性的毛状体特异性启动子显示在植物发育的所有阶段发挥作用。该启动子可以提供有效的生物工程，以提高害虫和病原体的抗性，并为基于毛状体腺体系统的分子农业。
Trichomes are specialized epidermal cells that produce secretions that are thought to provide a first line of defence against pests and pathogens. Many trichome-secreted compounds are used commercially as flavourings, medicines, etc. Described here is the cloning and characterization of the promoter of a tobacco trichome-specific P450 gene, CYP71D16. This promoter is shown to direct the specific expression of the reporter gene, beta-glucuronidase (GUS), in glandular trichomes of Nicotiana tabacum cv. T.I. 1068 at all developmental stages. With the full promoter, GUS activity was predominantly in the gland cell, with less in the stalk cell adjacent to the gland, and in lower stalk cells. GUS staining was also observed in the most distal trichome stalk cells of non-glandular trichomes found on variety T.I. 1112. Promoter deletion analysis revealed that the region from -223 to +111 bp is sufficient to direct trichome-specific expression, but not strong gland expression. Examination of the literature suggests that this is the first characterized trichome-specific-promoter shown to function at all stages of plant development. This promoter may provide efficient bioengineering to enhance pest and pathogen resistance, and for molecular farming based on the trichome gland system.