Monoclonal antibody to a 35 kD epidermal protein induces cell detachment.
Monoclonal antibody to a 35 kD epidermal protein induces cell detachment.
复制标题
针对 35 kD 表皮蛋白的单克隆抗体可诱导细胞脱离。
DOI：
10.1111/1523-1747.ep12275639
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Goldsmith,LA
中科院分区：
文献类型：
--
作者：
Negi,M;Lane,AT;McCoon,PE;Fairley,JA;Goldsmith,LA
用胰酶消化培养的人包皮角质形成细胞免疫BALB/c小鼠，制备了小鼠单抗(ECS-1)。该抗体在正常人表皮、成人手掌、小鼠嘴唇表皮和培养的人角质形成细胞的核层上显示出一种细胞间染色的模式。ECS-1染色的人胎儿皮肤，估计孕周为9周。ECS-1与从新生儿包皮表皮和培养的人角质形成细胞中提取的35kD蛋白反应。这种蛋白质需要NONIDT P-40或十二烷基硫酸钠和硫醇才能溶解。ECS-1可诱导表皮细胞脱离，补体可增强ECS-1的作用。ECS-1与人类天疱疮抗体具有相同的特征。
A murine monoclonal antibody (ECS-1) was prepared from BALB/c mice immunized with trypsinized cultured human foreskin keratinocytes. The antibody showed a pattern suggestive of intercellular staining on the nucleated layers of normal human epidermis, adult palm, mouse lip epidermis, and cultured human keratinocytes. ECS-1 stained human fetal skin by 9 weeks estimated gestational age. ECS-1 reacted with a 35 kD protein extracted from neonatal foreskin epidermis and cultured human keratinocytes. The protein required Nonidet P-40 or sodium dodecyl sulfate and mercaptoethanol for solubilization. ECS-1 induced epidermal cell detachment which was enhanced by complement. ECS- 1 shares characteristics with human pemphigus antibodies.