CATALYTIC ACTIVITY OF AN RNA MOLECULE PREPARED BY TRANSCRIPTION INVITRO
CATALYTIC ACTIVITY OF AN RNA MOLECULE PREPARED BY TRANSCRIPTION INVITRO
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DOI：
10.1126/science.6199841
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发表时间：
1984-01-01
期刊：
影响因子：
56.9
通讯作者：
ALTMAN, S
中科院分区：
文献类型：
--
作者：
GUERRIERTAKADA, C;ALTMAN, S
RNase P是一种核糖核蛋白，它切割tRNA分子的前体以产生成熟tRNA的正确5'末端序列。来自大肠杆菌的RNase P的RNA片段M1 RNA和体外制备的M1 RNA基因的未加工转录物都可以在缺乏该蛋白片段的情况下进行典型酶的裂解反应。当M1 RNA基因的转录本与蛋白质片段结合时，来自大肠杆菌的tRNA前体和4.5S RNA前体被切割。
RNase P is a ribonucleoprotein that cleaves precursors to tRNA molecules to yield the correct 5'' terminal sequences of the mature tRNA. The RNA moiety M1 RNA of RNase P from Escherichia coli and the unprocessed transcript prepared in vitro of the gene for M1 RNA can both perform the cleavage reactions of the canonical enzyme in the absence of the protein moiety. When the transcript of the M1 RNA gene is combined with the protein moiety, a tRNA precursor and the precursor to 4.5S RNA from E. coli are cleaved.