TURNIP CRINKLE VIRUS DEFECTIVE INTERFERING RNAS INTENSIFY VIRAL SYMPTOMS AND ARE GENERATED DENOVO
TURNIP CRINKLE VIRUS DEFECTIVE INTERFERING RNAS INTENSIFY VIRAL SYMPTOMS AND ARE GENERATED DENOVO
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DOI：
10.1073/pnas.86.23.9173
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发表时间：
1989-12-01
影响因子：
11.1
通讯作者：
SIMON, AE
中科院分区：
文献类型：
--
作者：
LI, XH;HEATON, LA;SIMON, AE
缺陷性干扰（DI）RNA已从广谱动物病毒中分离，并且最近已在植物病毒感染中鉴定。由于其普遍存在的性质，DI被认为在病毒复制和产量中起重要作用。现已发现DI RNA与芜菁皱缩病毒（TCV-B）的天然分离物相关，并且在用源自克隆转录物的病毒接种芜菁后从头产生DI RNA。在TCV-B分离株中天然发现的DI RNA G是具有5'和3'病毒片段以及在3'片段开始处的36个核苷酸的重复的嵌合分子。DI RNA G的5 '端21个核苷酸与基因组TCV序列不相似，但与在与TCV相关的其他小RNA（卫星RNA）的5'端发现的序列相似。DI RNA G干扰TCV基因组RNA的积累，并且与其他DI RNA不同，它会加剧其辅助病毒的症状。用TCV-B的克隆转录本衍生的病毒感染芜菁，导致从头产生DI RNA，DI 1 RNA。DI 1 RNA与DIRNA G的不同之处在于含有TCV的精确的5'和3'末端以及内部病毒片段。
Defective interfering (DI) RNAs have been isolated from a broad spectrum of animal viruses and have recently been identified in plant virus infections. Because of their ubiquitous nature, DIs are thought to play an important role in virus replication and yields. DI RNAs have now been found in association with a natural isolate of turnip crinkle virus (TCV-B) and are generated de novo after inoculation of turnip with virus derived from cloned transcripts. DI RNA G, naturally found in the TCV-B isolate, is a mosaic molecule with 5' and 3' viral segments and a repeat of 36 nucleotides at the beginning of the 3' segment. The 5'-terminal 21 nucleotides of DI RNA G were not similar to genomic TCV sequences but did resemble sequences found at the 5' end of other small RNAs associated with TCV (satellite RNAs). DI RNA G interferes with the accumulation of TCV genomic RNA and, unlike other DI RNAs, intensifies the symptoms of its helper virus. Infection of turnip with virus derived from cloned transcripts of TCV-B resulted in de novo generation of a DI RNA, DI1 RNA. DI1 RNA differed from DI RNA G by containing exact 5' and 3' ends of TCV as well as an internal virus segment.